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The aim of this experiment is to find out how the temperature effects the action of lipase.

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Introduction

Robynne Shipley 10G Factors Effecting Action of Lipase Effect of Temperature on the action of Lipase Aim The aim of this experiment is to find out how the temperature effects the action of lipase. Method * A glass beaker was taken and filled with about halfway with water. * The water was then heated to 50�c * Into a boiling tube 5 cm� of milk, 7 cm� of sodium carbonate and a few drops of Phenothalin were placed. * The boiling tube was placed into the water for a few minutes so it could warm to the temperature of the water. ...read more.

Middle

At 100�c I predict that there will be no reaction because the enzymes will be de-natured because it is so hot. And at 0�c I predict there will also be no reaction as it will be too cold for the enzymes to work. Variables * Amount of milk - 5 cm� * Amount of sodium Carbonate - 7 cm� * Amount of lipase - 1 cm� * Heat - 0�c, 13�c, 30�c, 50�c, 100�c Results Temperature (�c) 1st time (s) 2nd time (s) Average (s) 0 (No result) (No result) N/A 13 671 757 714 30 228 251 353 50 95 111 103 100 (No result) ...read more.

Conclusion

The results also show that at 50�c the reaction is the quickest and so therefore proved me wrong, as I believed that 30�c would be the temperature that the lipase reacted quickest at, but it wasn't. The experiment went well and some good results came from it. It would be interesting to try more different temperatures and so then a better more accurate graph would be achieved, it would also be interesting to find out the point at which the lipase is actually denatured (i.e. the highest temperature that the lipase can work at). And if I were to do further work on these experiments I would most definitely choose to go down that line of experiments. It would create some interesting results. ...read more.

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