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The determination of the water potential of potato tissue.
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--The determination of the water potential of potato tissue
This experiment involves the use of different sucrose solutions with different concentrations, in order to measure the water potential in a chosen potato.
The theoretical background is the osmosis in plant cells: a plant cell loses water by osmosis if it is in a more concentrated solution (which has a lower water potential) than the cell's cytoplasm and vacuole. Reversely, a plant cell absorbs water by osmosis if it is placed in a solution of higher water potential.
The difference of water potential between the cell's cytoplasm and vacuole and the surrounding solution causes water to enter or leave the cell, resulting the change of the tissue's mass.
1. Prepare 18 potato slides, which each has a thickness of 4 to 5 millimetres, and divide them into 6 groups. Blot dry, weigh on a balance and mark down their weight as ' First weight'.
2. Dilute the 10% sucrose solutions provided and thus provide six different sucrose solutions with concentrations of 10%, 5%, 2.5%, 1.25%, 0.6% and 0%.
3. Put the samples
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