Metals binds to the enzymes and distort the shape of the enzymes which active the site, therefore this slows the reaction.
The binds active site which therefore preventing the substrate form binding, this led to less reaction
Method
First I will measure 5cm of 2 moles of copper sulphate and add 5cm of diluted water. Than I will take out 5cm of that solution from that test tube and add it to another test tube that has already got 5cm of water. I will repeat till I have five test tubes and in the last test tube I have 0.025moles of copper sulphate left the extra 5cm I will throw it away.
Secondly I will take out 2cm from each test tube and add liver into the solution and put a bung on top. In washing up bowl I have put inside water. Inside the washing up blow there is measure cylinder upside down full of water with a pipe going from the measure cylinder to the test tube.
Lastly I will inject H2O2 (hydrogen peroxide) in the test tube and start timing and record every 30 seconds until no more oxygen can be produced.
Diagram
Results
Conclusion
In my conclusion I found out that my prediction was half wrong as you can see from the results table, because in 2 moles of CuSO4 solution 11cm of oxygen was produce so some where in the experiment it went wrong because should have been 5cm. But is I had repeated the experiment than that would have given me average set of results.
Evaluation
The way I could have made my experiment better was to have repeated the test/experiment so it would have given be an average set of results. Than every thing that I had said in my prediction would have been correct.