• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month
Page
  1. 1
    1
  2. 2
    2
  3. 3
    3
  4. 4
    4
  5. 5
    5
  6. 6
    6
  7. 7
    7
  8. 8
    8
  9. 9
    9
  10. 10
    10
  11. 11
    11
  12. 12
    12
  13. 13
    13
  14. 14
    14

The Effect of Different Concentration of Hydrogen Peroxide on the Rate of Reaction of Catalase Enzyme.

Extracts from this document...

Introduction

The Effect of Different Concentration of Hydrogen Peroxide on the Rate of Reaction of Catalase Enzyme. Made By: Ismail Mohamed Essam Haggag , 4/7 PLANNING Introduction An enzyme is a protein that acts as a catalyst. They are made in all living cells. A catalyst is a chemical substance which speeds up a reaction but does not get used up during the reaction or altering its end products. One enzyme can be used many times over. The substance that enzymes act on is the substrate. The substance formed by the reaction is the product. The site on which the enzyme takes place is called the active site. Each enzyme has an active site which fit the type of substrate it works on forming enzyme-substrate complex. The reaction then takes place and the end product separate from the enzyme. The enzyme is then the free substrate molecule. The enzyme that acts on a certain type of substance will not act on a different one, for example an enzyme that act on protein does not act on starch. There are two types of reactions in the enzymes; building up reactions and breaking down reactions. ...read more.

Middle

using a pipette. 7) I will dip a piece of filter paper in each test tube. 8) Using a digital stopclock, I will record the time from when the filter paper reaches the bottom of the test tube till it starts to rise up again, this will be repeated for each test tube. 9) I'm going to repeat the experiment three times for each test tube (to make sure the results are reliable), take three readings and take the average time for each test tube; this will be my records that I'm going to work on. Precautions While doing the experiment we have to be careful in: 1) Using some tools or to be aware avoiding the solutions which may be harmful to sensitive parts such as the mouth or the eyes. 2) Using the scalpel. 3) Wearing gloves to avoid the contact with harmful substance. 4) Washing the test tubes well with water and dry it in order not to affect the solution that will be used in it. 5) Cutting the filter paper into equal pieces not to affect the rate of the enzyme. 6) Using the stopclock accurately, and in watching the filter paper while touching the bottom and start rising up. ...read more.

Conclusion

Moreover, I carried out the experiment 3 times for each concentration of Hydrogen Peroxide (2.0 mol, 1.5 mol, 1.0 mol, and 0.5 mol) and I calculated the average of the 3 experiments for each concentration. From my results and graphs I think that I have enough evidence to draw a firm conclusion that there is a relational between the concentration of hydrogen peroxide and the rate of reaction of enzyme catalase. This means that increasing the concentration will decrease the reaction time and this is illustrated in the graphs. So as to improve my method to obtain more evidence to support my conclusion, I suggest to increase the concentration of hydrogen peroxide more than 2 mol and observe the results. On the other hand, I also believe that increasing the concentration gradually will reach a point above which there will be no further decrease in reaction time and the reaction rate will become stable. I will also try to apply the same experiment on other types of plant cells other that potato to observe the results such as carrot or beetroot and also apply on animal cells to compare between the different results. 13 ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Life Processes & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Life Processes & Cells essays

  1. Marked by a teacher

    Plan: The effect of the end product, phosphate, on the enzyme phosphatase

    5 star(s)

    Repeats of the experimental: To ensure that all the reactions are carried by the same enzyme, the enzyme suspension added to each test tube must from the same extract of potato. So the amount of enzyme suspension will limit the number of repeat experiments.

  2. Marked by a teacher

    To investigate the effect of substrate concentration on Catalase Activity.

    3 star(s)

    The results therefore were all rendered anomalies. Conclusion of trial run From the trial run I have concluded that we will need to find an appropriate bung and also find a device to hold the measuring cylinder within the water.

  1. Investigating the effect of enzyme catalase concentration on hydrogen peroxide.

    When, the apparatus was fully set up and active, the stopwatch was started. Also, the temperature and pH were measured and kept constant before each reading was taken. The timing measured how long the first bubble took to appear and then how many followed the first over the next two minutes.

  2. Factors affecting the activity of potato catalase on hydrogen peroxide.

    which is all they need, but if one of these factors stops or changes it can it can change our results because the enzymes would stop working and start to die, this would have a major effect on our experiment.

  1. Investigating the Effect of Enzyme Concentration on Rate of Reaction.

    I felt that a gas syringe was not very easy to use in my pilot. There was already gas in the end, which could vary between measurements - so an inconsistent error, which could lead to unreliable results. Also, for small volumes of gas evolved, the markings for measuring were imprecise, which could also lead to poor results.

  2. Investigating an enzyme-catalyzed reaction

    In preliminary I found that the delivery tube that I was using didn't work because it wasn't clean so we made sure before I used it we cleaned. We also found that all other equipment that we expected to use worked well and was successful.

  1. What influence does pH have on the enzyme Catalase?

    For these reasons we cannot put complete faith in the conclusion. Scientific Explanation of Conclusion However, the science explains the current conclusion. The reason pH effects the rate of enzyme reaction is because of how it can change the shape of the enzyme.

  2. Investigating the Effect of Substrate Concentration on Catalase

    This graph shows the rate of reaction with increasing substrate concentration. The substrate concentration is directly proportional to the initial rate of reaction. This is because when the substrate concentration doubles, so does the amount of oxygen that it decomposes into.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work