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The Effect of Different Concentration of Hydrogen Peroxide on the Rate of Reaction of Catalase Enzyme.

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Introduction

The Effect of Different Concentration of Hydrogen Peroxide on the Rate of Reaction of Catalase Enzyme. Made By: Ismail Mohamed Essam Haggag , 4/7 PLANNING Introduction An enzyme is a protein that acts as a catalyst. They are made in all living cells. A catalyst is a chemical substance which speeds up a reaction but does not get used up during the reaction or altering its end products. One enzyme can be used many times over. The substance that enzymes act on is the substrate. The substance formed by the reaction is the product. The site on which the enzyme takes place is called the active site. Each enzyme has an active site which fit the type of substrate it works on forming enzyme-substrate complex. The reaction then takes place and the end product separate from the enzyme. The enzyme is then the free substrate molecule. The enzyme that acts on a certain type of substance will not act on a different one, for example an enzyme that act on protein does not act on starch. There are two types of reactions in the enzymes; building up reactions and breaking down reactions. ...read more.

Middle

using a pipette. 7) I will dip a piece of filter paper in each test tube. 8) Using a digital stopclock, I will record the time from when the filter paper reaches the bottom of the test tube till it starts to rise up again, this will be repeated for each test tube. 9) I'm going to repeat the experiment three times for each test tube (to make sure the results are reliable), take three readings and take the average time for each test tube; this will be my records that I'm going to work on. Precautions While doing the experiment we have to be careful in: 1) Using some tools or to be aware avoiding the solutions which may be harmful to sensitive parts such as the mouth or the eyes. 2) Using the scalpel. 3) Wearing gloves to avoid the contact with harmful substance. 4) Washing the test tubes well with water and dry it in order not to affect the solution that will be used in it. 5) Cutting the filter paper into equal pieces not to affect the rate of the enzyme. 6) Using the stopclock accurately, and in watching the filter paper while touching the bottom and start rising up. ...read more.

Conclusion

Moreover, I carried out the experiment 3 times for each concentration of Hydrogen Peroxide (2.0 mol, 1.5 mol, 1.0 mol, and 0.5 mol) and I calculated the average of the 3 experiments for each concentration. From my results and graphs I think that I have enough evidence to draw a firm conclusion that there is a relational between the concentration of hydrogen peroxide and the rate of reaction of enzyme catalase. This means that increasing the concentration will decrease the reaction time and this is illustrated in the graphs. So as to improve my method to obtain more evidence to support my conclusion, I suggest to increase the concentration of hydrogen peroxide more than 2 mol and observe the results. On the other hand, I also believe that increasing the concentration gradually will reach a point above which there will be no further decrease in reaction time and the reaction rate will become stable. I will also try to apply the same experiment on other types of plant cells other that potato to observe the results such as carrot or beetroot and also apply on animal cells to compare between the different results. 13 ...read more.

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