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The Effect of Different Concentrations of Sucrose On Visking Tubing

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Preliminary Work: I am going to investigate the effect of different concentrations of sucrose on visking tubing Apparatus: * Measuring balance (used to measure the weight of the visking tubing) * 18 beakers (containing varying concentrations of sucrose) * 18 Visking tubing (containing sucrose solution of unknown concentration) Diagram: Method: * I will gather the equipment in my apparatus list * I will measure the weights of the visking tubing before they are put in the beaker. I will record the results * I will put the visking tubing's in the beakers and leave them to react overnight * I will take out the visking tubing and dry them, so no liquid will be left on it to effect the weight. I will then measure its weight on the measuring balance and record these results. * I will then work out the mass and percentage change Results: Solution (ml) Number Original mass (g) Final mass (g) Mass change (g) Percentage change (%) 0.0 1 2 3 Average 0.2 1 2 3 Average 0.4 1 2 3 Average 0.6 1 2 3 Average 0.8 1 2 3 Average 1.0 1 2 3 average Aim: To investigate the effect of osmosis in plant cells Apparatus: Electric Balance, Borer, Scalpel, Potato, Test tubes and racks, Ruler, Paper Towel Safety Goggles, ...read more.


Water and Sucrose are present in potato cells, our potato cells being of fixed 8mm diameter as that is diameter of the borer. The potatoes are protected by a partially permeable membrane, which allows small molecules to pass into it, such as water, but not larger molecules like Sucrose. Therefore, only water can pass in this reaction. Safety: For a fair experiment, and to be safe, safety procedures have to be performed. Some safety methods, that I will perform, are to wear safety goggles, whilst observing the reaction. Also, I will use safety mats, in case any of the solution will splash onto the table. Finally, I will use rubber gloves at all times, so my hands will not be covered in the solution, when I am taking the potato out of the beaker. Variables: Controlled variables * Concentration of sucrose in the potatoes, * The diameter is fixed at 8mm * The temperature is constant * Time is a constant Uncontrolled variables * Concentration of sucrose in solution * Weight of potato Making it fair: Each concentration of the solution, will be done three times, and there will be an average, to improve accuracy. There will be 6 different solutions, (0.0M, 0.2M, 0.4M, 0.6M, 0.8M, 1.0M). ...read more.


The shape of my graph shows definite correlation, but not direct proportionality. I believe that it should of being directly proportional but, my results were inaccurate in some places. All my points are close to the line of best fit, but they are not all on it. This shows that my experiment was quite accurate, but not accurate enough so they are all on the line of best fit. I had some slightly anomalous results and circled them on the graph. The reasons for the anomalous results could be; * The timing of the experiment could of being different for each potato affected the results, * Parallax error could be committed when cutting the potato. * There may be different concentrations of sucrose in different cells of the potato, * I could further my work, by weighing the test tubes to see how much they have gained or lost, so I can measure both the potato and the test tube to fin the mass change. * I could also further my work, by using different organic cells, like animal cells etc. My graph does support my conclusion as I mentioned in my analysis. I said that the small concentrations lose weight, the middle concentrations stay the same, and the high concentrations gain weight. This statement was correct as that is what happened. Alan Carrara 11T Osmosis- Biology Coursework ...read more.

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