• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

The Effect of Enzyme and Substrate Concentrations On the Rate of a Reaction

Extracts from this document...


The effect of enzyme and substrate concentrations on the rate of a reaction Requirements: CARE: Eye protection must be worn. * burette (50cm ) * trough or bowl * boiling tube with bung and delivery tube * graduated pipette (5cm ) and safety filler * measuring cylinder (10cm ) * hydrogen peroxide solution, 5 vol. (25cm ) * yeast suspension (20cm ), made from 2g dried yeast in 160cm water aerated for several hours * stopwatch Introduction: In this experiment I will follow the progress of a catalysed reaction by measuring the volume of gas produced as the reaction proceeds. I will use the initial rates of a series of experiments to try and find the orders of reaction with respect to enzyme and substrate. The enzyme in this reaction will be catalase, and the substrate will be hydrogen peroxide (H O ). Catalase is an enzyme and so catalyses the decomposition of hydrogen peroxide, thus producing water and oxygen. This reaction is written as a balanced equation as follows; 2H O (aq) - 2H O(l) ...read more.


After organising ourselves into taking turns in recording readings of volume at 10 second intervals for 4 minutes. 4: Then I added 5cm of the hydrogen peroxide to the yeast suspension and by quickly replacing the bung to the boiling tube, began the stopwatch as the first bubble reached the top of the burette. Recording the volume of gas in the burette every 10 seconds for 4 minutes was relatively straightforward. 5: After washing out the boiling tube, and refilling the burette with 50cm of water, I repeated steps 1-4 four more times using the following; 4cm H O + 1cm distilled H O 3cm H O + 2cm distilled H O 2cm H O + 3cm distilled H O 1cm H O + 4cm distilled H O in the measuring cylinder. Everything else was kept the same in each of the experiments in order to make sure that the test was a fair one. 6: Finally I plotted a graph from my results, with the volume of O given off on the vertical y axis, against time on the horizontal x axis for each experiment. ...read more.


There only explanation that I can account for is the probability that there was some extra H O present or maybe a slight difference in the volume of enzyme. Another common problem is that the person whom is recording the data may have made a slight misjudgement. From part two in the experiment, where the volume of yeast was altered in ratio to the distilled water, the results were much more straightforward, and fitted the expected outcome. Thus being that the less yeast added along with the more distilled water, the volume of O given off was decreasingly less. The differences amongst the separate experiments was quite large, as the amount of yeast obviously had a big impact upon the oxygen. I have learnt from doing this experiment that you should always make sure you have the absolute correct measurements, and that the conditions are all kept at a constant to ensure a fair test, and hopefully disable any strange results. I will now construct two graphs, one for each part of the experiment. This should give a better indication of the visual effect that the variables whence changed, have an impact upon the outcome of the volume of oxygen given out. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Patterns of Behaviour section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Patterns of Behaviour essays

  1. Investigate the effect of changing substrate concentration on the rate of the reaction between ...

    Also, the tubing was sometimes inserted too high up into the test tube and in the middle of the reaction, we had to rearrange the apparatus so as to keep the tubing immersed in the water and keep up the production of oxygen bubbles.

  2. Enzymes - show how substrate concentration affects the rate of reaction for an enzyme ...

    As result of this particles move faster and faster moving particles collide more frequently. This will result in more product, water and oxygen produced in a given time. Hence rate of reaction will increase as temperature increases. Use a thermostatically controlled water bath to maintain the constant temperature of 35�C


    Thermometer Ensure temperature of solutions was at 35�C The thermometer was used so that the experiment would be reliable enabling you to see the temperature and ensuring was at 35�C. I needed to thermometers one for the bile and one for the lipase to avoid cross contamination.

  2. The Effect of Catalase in the Breakdown of Hydrogen Peroxide

    (cm3): Volume of Oxygen: Initial Reading (cm3): Volume of Oxygen: AVERAGE Initial Reading: AVERAGE Volume of Oxygen: 0 49.7 0 47.7 0 48.7 0 2 47.9 1.8 47.3 0.4 47.6 2.2 4 45.9 3.8 45.4 2.3 45.7 3.1 6 44.2 5.5 44 3.7 44.1 4.6 8 43.4 6.3 43.4 4.3

  1. Investigation into the effect of substrate concentration on the rate of hydrogen peroxide decomposition ...

    apart from that on the neck of the flask a piece of glass tubing is already attached. This will allow the gas syringe to be connected via a length of rubber pipe of a suitable diameter. A normal rubber bun could then be used to seal the opening of the flask.

  2. An investigation to establish how concentration of substrate effects the rate of anaerobic respiration ...

    I have chosen 37.5�c because this is the optimal temperature for enzyme activity, which is pivotal in the rate of anaerobic respiration (see theory for details). 2. Yeast concentration. This variable also has the potential to destroy any accuracy and reliability that my data would be able to show me.

  1. The effect of concentration on the activity of catalase.

    Apparatus with justification: 1. Potato peeler --> Will be used to remove the skin of the potato. 2. Knife -->Will be used to cut the potato into cubes of the necessary quantity needed. 3. Ruler -->Will be used to measure a cm3 for each potato cube.

  2. Effect Of Substrate Concentration On The Activity Of Catalase

    I am using yeast catalase as opposed to catalase from apples, potatoes or liver because it is easier to get the desired amount of yeast catalase by simply measuring it off. To obtain catalase from a substance such as potato would involve crushing it and with that method you would never be sure of the concentration of the catalase.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work