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The effect of salinity on seed germination

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Introduction

Experiments: the effects of soil salination The effect of salinity on seed germination Materials * Seeds of two plant varieties (e.g. beans, sunflower) * 10 Petri dishes * Cotton wool * Dropper bottles (5) containing the following: * distilled water (control) * 0.25 grams salt per litre distilled water * 0.50 grams salt per litre distilled water * 1.0 grams salt per litre distilled water * 2.0 grams salt per litre distilled water * Plastic film Method 1. Place cotton wool in the bottom of each Petri dish. 2. Label one set of 5 dishes A1 to E1; label the remaining 5 dishes A2 to E2. 3. Spread seeds of one variety across the cotton wool in one set of 5 Petri dishes. Repeat for the second seed variety in the other set of dishes. ...read more.

Middle

4. How did the two kinds of seeds differ in their response to the salt solution? Point out any differences between them. 5. What are the implications for farmers in salt-affected areas? The effect of salinity on plant growth Materials * Ruler * Fast-growing plants that have reached an advanced stage of germination (e.g. beans, sunflower) * Coarse washed sand * 5 plastic plant pots (15 cm in diameter) * Measuring cylinders * Beakers * 20 litres non-saline nutrient solution (`Aquasol' or similar, mixed as per instructions; ensure micro-nutrients are included) * Dropper bottles containing 5 salt/nutrient solutions at the following concentrations: * nutrient solution only (control) * 0.25 grams salt per litre nutrient solution * 0.50 grams salt per litre nutrient solution * 1.0 grams salt per litre nutrient solution * 2.0 grams salt per litre nutrient solution * Foil Method 1. ...read more.

Conclusion

Measure plant growth (height of stem above soil) at regular intervals. Note events such as appearance of new leaves, and any strange symptoms on any leaves. Use table 1 to record your observations. 5. At the end of 2--3 weeks, when obvious differences have occurred in the treatment plants, harvest the trial and dry and weigh the shoots. Results and analysis Table 1 Solution (salt conc.) Observations Amount of solution New leaves Leaf appearance Harvested dry weight (relative yield) Week 1 Week 2 Week 3 I O I O I O control 0.25 g/L 0.50 g/L 1. g/L 2. g/L I = in (mL solution added) O = out (mL solution drainage) 1. Describe your results. 2. Is there a relationship between the amount of water used and the salinity of the solution? Explain. 3. Plot on separate graphs: a. the harvested dry mass (relative yield) against salinity b. the stem height against salinity. ...read more.

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