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The Effect of Surface Area on Catalase Activity in Potato

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Introduction

The Effect of Surface Area on Catalase Activity in Potato Hypothesis: In this investigation, I will be trying to find out how the surface area of a potato will affect the rate of reaction, but I will be keeping the rest of the variables constant, (pH, substrate concentration, temperature and inhibition). The only variable that I will not be keeping constant is the enzyme concentration, which is the potato i.e. the size of the cubes. The way I will carry out my experiment is that I will first put 10cm^3 of hydrogen peroxide in five different test tubes each. Then I will cut out five different sized potato cubes. The first potato cube will have a surface area of 6cm^2; I will do this by cutting a potato cube in the shape of a cube of a cube and each side of the potato surface area will be 1cm^2. The second surface area will have a surface of 24cm^2; I will do this cutting a potato in the shape of a cube with each surface area of the cube at 4cm^2. The third surface that I will do is 54cm^2; I will do this by cutting a potato cube in the shape of a cube of a cube and each side of the potato surface area will be 9cm^2. The fourth surface that I will do is 96cm^2; I will do this by cutting a potato cube in the shape of a cube of a cube and each side of the potato surface area will be 16cm^2. ...read more.

Middle

Rate of reaction Rate at which reaction increases due to increased kinetic energy of substrate and enzyme molecules Temperature Rate of reaction Rate of reaction Temperature 40^0C Rate at which reaction decreases due Temperature To denaturisation of the enzyme Actual rate of reaction as a result of the combined effect of the other 2 influences pH: The precise three-dimensional shape of enzymes, which, is vital for the enzymes to function is partly due to hydrogen bonding. These bonds may be broken by the presence of hydrogen ions (H^+) present. pH is a measure of hydrogen ion concentration. It is measured on a scale of 1-14, with pH 7 being neutral. A pH below 7 is an acid and a pH above seven is an alkaline (base). The change of one point of pH represents a change in hydrogen ion concentration. Compared to water, which has a pH of 7, a solution which has a pH of 6 will be ten times more acidic, a solution of pH 5 will be a hundred times more acidic, a solution with a pH of 4 will be a thousand times more acidic and so on. By breaking the hydrogen bonds, which give enzymes their shape, any change in pH can effectively denature enzymes. Each enzyme works efficiently at a particular pH, and any change from this optimum may result in denaturisation of the enzyme. The graph shows different pH optima of 4 different enzymes. Rate of reaction Pepsin Sucrase Salivary amylase Arginase 2 4.5 7 10 pH Inhibition: The rate of enzyme-controlled reactions may be decreased by the presence of inhibitors. ...read more.

Conclusion

Inhibition: In this experiment no inhibitors will be added so this will not affect the rate of reaction. Method: First you should start by collecting all the equipment listed. Then you should set up the diagram as shown on the diagram. After making sure everything is set up correctly, use the cork bore to cut the different sized potato cubes. Then you should put 10cm^3 of hydrogen peroxide and 2cm^3 buffer solution into a test tube. Thereafter put the first potato cube in the test tube. As soon as you drop the potato in, you must close the bung properly in order to make sure that no oxygen escapes. You must also make sure to start the stopwatch. Then, measure the amount of oxygen in the measuring cylinder and the more oxygen there is the faster the rate of Catalase activity and the slower the rate of Catalase activity, the lower the amount of oxygen being produced. The experiment should be repeated at least 3 times for the accuracy of results and so that it is a fair test. Health & Safety: Firs of all, because there is going to use of hydrogen peroxide (which is corrosive), goggles, gloves and a lab coat must be worn. I will use a safety tile so that the potato or bench aren't going to be in any danger of germs. I will also have to consider other people because I will be working alongside my fellow classmates, so I will have to be aware of other people's movements. The main term if safety is to be sensible and to act quickly if any spillage of hydrogen peroxide was to occur. ...read more.

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