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The effect of temperature change on the activity rate of Trypsin.

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The effect of temperature change on the activity rate of Trypsin Introduction This piece of coursework will look at what effect temperature has on the activity of Trypsin and which temperature Trypsin works best at. It was predicted that the milk would turn transparent quickest at the 40 oC temperature level, as the enzyme in a Trypsin is found in human bodies. The normal human body temperature is around 37 oC so the activity of the enzyme would work best at this temperature. Method A total mixture capacity of 10ml mixture Trypsin and milk, of 5ml of each, 5ml syringes were used to get the exact amount each time. The temperature range of 60 oC to use for the experiment, between 10 oC and 70 oC using 10 oC increments. ...read more.


Preliminary testing took place to establish that the experiment would perform as expected, with modifications to the method if required. This is the results of the preliminary test. Temperature Times in seconds 10 oC 421 20 oC 133 30 oC 102 40 oC 82 50 oC 143 60 oC 159 70 oC 181 During the preliminary testing it was noticed that the temperature of the separate test tubes of liquids did not always reach the required temperature within 1 minute. It was decided to increase the time allowance to 2 minutes for the separate test tubes of Trypsin and milk get to the correct temperature before the were mixed together. The equipment used for the experiment Equipment Number Test Tube 45 Test Tube rack 1 5ml syringe 2 100ml beaker 1 Water bath (Ice cream container) ...read more.


times in seconds Temperature attempt 1 attempt 2 attempt 3 average 10 oC 378 458 480 439 20 oC 133 129 117 126 30 oC 118 98 102 106 40 oC 69 81 87 79 50 oC 103 171 155 143 60 oC 164 158 140 154 70 oC 173 147 212 187 Analysis The collected data is presented as a graph, showing the average time the mixture took to change to transparent against temperature over the 60 oC range. Each temperature was repeated three times so that an average time could be established, this would also show up gross errors in the experiment. A line of best fit was used on the graph. Conclusion The graph clearly shows that for this experiment Trypsin's activity rate is quickest just below the 40oC temperature. We know this because the mixture of milk and Trypsin went transparent quickest at 40oC. ...read more.

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3 star(s)

This is a good write up of a practical. It is clear and concise and uses diagrams to aid clarity.

It could be enhanced by including some background research and extending the conclusion to explain why.


Marked by teacher Sam Morran 15/05/2013

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