• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

The effect of temperature change on the activity rate of Trypsin.

Extracts from this document...


The effect of temperature change on the activity rate of Trypsin Introduction This piece of coursework will look at what effect temperature has on the activity of Trypsin and which temperature Trypsin works best at. It was predicted that the milk would turn transparent quickest at the 40 oC temperature level, as the enzyme in a Trypsin is found in human bodies. The normal human body temperature is around 37 oC so the activity of the enzyme would work best at this temperature. Method A total mixture capacity of 10ml mixture Trypsin and milk, of 5ml of each, 5ml syringes were used to get the exact amount each time. The temperature range of 60 oC to use for the experiment, between 10 oC and 70 oC using 10 oC increments. ...read more.


Preliminary testing took place to establish that the experiment would perform as expected, with modifications to the method if required. This is the results of the preliminary test. Temperature Times in seconds 10 oC 421 20 oC 133 30 oC 102 40 oC 82 50 oC 143 60 oC 159 70 oC 181 During the preliminary testing it was noticed that the temperature of the separate test tubes of liquids did not always reach the required temperature within 1 minute. It was decided to increase the time allowance to 2 minutes for the separate test tubes of Trypsin and milk get to the correct temperature before the were mixed together. The equipment used for the experiment Equipment Number Test Tube 45 Test Tube rack 1 5ml syringe 2 100ml beaker 1 Water bath (Ice cream container) ...read more.


times in seconds Temperature attempt 1 attempt 2 attempt 3 average 10 oC 378 458 480 439 20 oC 133 129 117 126 30 oC 118 98 102 106 40 oC 69 81 87 79 50 oC 103 171 155 143 60 oC 164 158 140 154 70 oC 173 147 212 187 Analysis The collected data is presented as a graph, showing the average time the mixture took to change to transparent against temperature over the 60 oC range. Each temperature was repeated three times so that an average time could be established, this would also show up gross errors in the experiment. A line of best fit was used on the graph. Conclusion The graph clearly shows that for this experiment Trypsin's activity rate is quickest just below the 40oC temperature. We know this because the mixture of milk and Trypsin went transparent quickest at 40oC. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Green Plants as Organisms section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Here's what a teacher thought of this essay

3 star(s)

This is a good write up of a practical. It is clear and concise and uses diagrams to aid clarity.

It could be enhanced by including some background research and extending the conclusion to explain why.


Marked by teacher Sam Morran 15/05/2013

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Green Plants as Organisms essays

  1. Marked by a teacher

    Determine the effect that temperature has on the rate of diffusion of food coloring ...

    coloring to diffuse completely within the beaker, was slightly slower than that of the food coloring in the beaker containing warm water. It is in this aspect that the hypothesis regarding diffusion in cold water was correct. When the water was heated to a warm temperature, in this case 50C,

  2. Peer reviewed

    An Investigation into the Effects that Different Light Intensities have on the Speed of ...

    5 star(s)

    the video recorder and the paths of the 5 different woodlice were followed one at a time using the coloured pens. A different coloured pen was used for each woodlouse in order that the paths could be distinguished easily, especially as there was a great deal of crossing over.

  1. Factor affecting the rate of fermentation.

    36.00 18 79.00 18 60.00 18 27.00 18 37.00 19 82.00 19 63.00 19 29.00 19 38.00 20 85.00 20 66.00 20 30.00 20 39.00 They all start at 10mL (be more accurate). The temperature was 41?, yeast at 3g and two drops of NaHCO3 (1M).

  2. Determine the water potential of potato tuber cell with the varying affect of solute ...

    I made this good prediction because I knew that even though the concentration of water was still more on the outside than on the inside if the cell, it had gone down a lot due to the increase in solute concentration.

  1. What is the effect on the rate of respiration of yeast cells with glucose ...

    take place faster but if it is not active it is slow, as the removal of hydrogen from the pathway takes longer. This factor could not be controlled Fair Test Whilst carrying out the experiment I had to show an awareness of the factors that I could control in order to try and maintain a fair test.

  2. Experiment to Investigate the Effect of Temperature on the Rate of Photosynthesis in Elodea.

    Analysis The graph shows that as the temperature increases up to a point, so does the amount of oxygen produced. From this maximum point, the amount of oxygen produced decreases with temperature up to the point where no more oxygen is produced.

  1. What effect does the sucrose concentration have on osmosis?

    Method Apparatus Apparatus: * Potato * Scalpel * Ruler * Test Tubes * Sucrose Solution * Measuring Cylinder * Distilled Water * Cutting plate Experiment Instructions: 1. Firstly I will make sure that all the potato chips weigh roughly the same.

  2. The relationship between flow rate and temperature difference

    The basic relationship for determining the liquid's flow rate in such cases is: Q = V x A where Q = liquid flow through the pipe V = average velocity of the flow A = cross-sectional area of the pipe Specific heat capacity (SHC)

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work