• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

The effect of temperature on amylase activity.

Extracts from this document...

Introduction

Biology coursework: effect temperature on enzyme activity Aim: I have been asked to investigate the effect of temperature on amylase activity. Prediction: I think that as the temperature is increased, the reaction rate will be increased. This is because as the temperature rises the molecules will move faster and have an increased chance of collision and the rate of reaction will be increased. If I was using human amylase from the mouth, I would predict that it would work best at around 37�C because this is the average temperature of the human body and the temperature the enzyme would work best at. However, as the Amylase I am using is from a laboratory and it is stronger, I think it will work best at around 40�C or higher. After this at around 60�C-80�C, the rate will start to decrease as the enzymes become denatured. Method: 1. Fill spotting tile with 3 drops of iodine in each well. 2. Fill a test tube with 10ml of starch and add 2ml of Amylase. ...read more.

Middle

To do this I will measure the amounts with a measuring cylinder carefully. I will also keep the volume of iodine I put in each well the same and the volume of starch and amylase I put into each well the same because if there were different amounts it could affect the reaction rate. The variable I am going to change is temperature. This is because the aim of this investigation is to find out what effect temperature has on the rate of Amylase activity. Safety: To make sure my experiment is safe I will have to ensure that: -long hair is tied back so it does not get in the way. -protection goggles are worn when using Amylase as it is harmful to eyes. -bags and stools are tucked under the tables so nobody is to trip. -precaution is taken with hot water so nobody is to scold themselves. Measuring results: I will measure my results every 30 seconds using a pipette to put 3 drops of starch and amylase solution into the iodine until there is no colour change. ...read more.

Conclusion

This was correct because my results show that the amylase worked best at 40�C and started to denature after that. If this was human amylase it would have worked best at 37�C and denatured at a lower temperature. Evaluation: The experiment went well and was quite successful. I had reliable results and a good range of readings that show how well amylase breaks down starch at different temperatures. My results had no anomalies or unusual readings so they were strong enough to support my conclusion. I could improve my experiment by taking more readings or taking them at closer intervals to get a wider range of results. I could have also used a different pipette each time I put new drops of the starch and amylase solution into the spotting tile to ensure that there was no solution left on it from the previous sample. Another thing that could have affected my results is the timing, human error can occur when starting and stopping the stop-clock and this could make slight difference in my results. For this experiment I could have also investigated varied concentrations of the amylase and/or starch solution, or varied the volumes of amylase and/or starch. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Patterns of Behaviour section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Patterns of Behaviour essays

  1. Rates of Reaction - The Iodine Clock

    [H2O2 ] z = Order w.r.t. [ KI ] Apart from modifications previously noted, I found no other reason to modify my experimental method during the course of the investigation. RESULTS 1 Concentrations for set: Varying concentration KI Na2S2O3 0.025M KI 0.1M H2O2 20 Volume H2SO4 1M TRIALS: Volume (ml)

  2. Investigate the effect of temperature on amylase activity.

    *results: Temp (�C) Time taken (mins) Average (mins) 1 2 3 3 (ice water) 8.30 9.30 9.00 8.86 18 (room temp) 6.30 7.00 7.00 6.76 20 5.30 5.30 5.00 5.20 40 5.30 5.00 5.00 5.10 50 6.00 6.00 5.30 5.76 70 12.00 12.00 12.00 12.00 80 12.00 12.00 12.00 12.00 Conclusion: the amylase

  1. Activity of Diastase On Starch

    ml Mean volume of reaction mixture used = 8.6+8.6+8.5 = 8.57 ml 3 Effect of change in temperature on the rate of hydrolysis of starch. Temperature Volume of reaction mixture used Rate= 1/volume of reaction mixture used 20�c 6.44 ml 0.155 30�c 5.57 ml 0.180 40�c 6.23 ml 0.161 70�c

  2. Free essay

    Close Your Eyes

    He jumped back up on top of the table and we got right to it. His body moved over mine and I held on to his sides, guiding him backward and forward. The table shook as he began driving in harder, I called out his name and he became faster and harder again.

  1. The Iodine Clock

    repeat all of the experiments because there seemed to be no anomaly results and so there was no need. There is enough evidence to prove that as the thiosulphate concentration increases so does the time in which it takes for the solution to turn purple, you can see this by

  2. The Effect of Temperature on the Reaction Between Amylase and Starch

    The first half of the results (up to about 50 C) were as expected, but instead of denaturing at higher temperatures, the enzyme seemed to work even faster, creating a few anomalous results. This was probably down to the fact that I did not heat the amylase before using it

  1. THE EFFECT OF BILE SALT ON THE ACTION OF THE ENZYME LIPASE

    the level only dropped by 2, however, if compared to 5%, this fall in pH increased to 2.8, which proves that bile has an affect on the breakdown of fats in the cream. However, to further support this claim and gain clearer evidence of whether or not bile affects the

  2. To investigate and compare the effect of temperature on the activity of catalase in ...

    Also to help with ensuring the same surface area for the tissues. White tile To ensure hygiene when cutting the tissues especially the animal tissue Blender To ensure the liver and potato pieces are of similar surface area. Syringe To ensure the correct amount of hydrogen peroxide is used each

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work