• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

The effect of temperature on membrane permeability

Extracts from this document...

Introduction

The effect of temperature on membrane permeability Aim: This experiment aims to determine what affect an increase in the surrounding Temperature has on the plasma membrane of a typical plant cell structure. Hypothesis: An increase in temperature will damage and denature the proteins, which are Within plasma membrane and cause the cytoplasm and other substances Contained within the membrane to leak out. Introduction: The function of a cell membrane is to control the transport of substances moving Into and out of a cell. The membrane is an extremely thin layer 8 to 10 manometers (nm) thick, which is partially permeable. It consists mostly of lipids and proteins. The lipids found in cell membranes belong to a class known as triglycerides, so called because they have one molecule of glycerol chemically linked to three molecules of fatty acids. The majority belong to one subgroup of triglycerides known as phospholipids. In the cells of a beetroot plant, a substance called beta cyanin is contained within the plasma membrane. It is beta cyanin, which gives the beetroot its characteristic blue/purple colour. If a cell is damaged in a beetroot plant and the membrane is broken, the beta cyanin leaks from the cells like a dye. ...read more.

Middle

With the use of green filter, green light will only be absorbed and the red colour of beta cyanin would reflect. 13. Calibrate the colorimeter using distilled water as a blank and then take the reading of your 7 solutions in % absorption units. Use the same colorimeter tube for all the 7 readings. Results Temperature �c % of absorption 70 0.28 60 0.20 50 0.12 40 0.07 30 0.06 24 0.08 -5 0.45 Conclusions: After collecting and correlating the results, I have come to the conclusion that the experimental hypothesis is correct in that an increase in temperature will damage and denature the plasma membrane and cause the cytoplasm and other substances contained within the membrane to leak out. This has been shown by a steady decrease in beta cyanin leaked out of plant cells as the temperature increases referring to the % of absorption. The graph can be analysed into three parts, first part there is a rapid decrease of the % of absorption from -5�c to 24�c which is the room temperature. From the data that are shown beetroot has the darkest % of absorption at -5�c . ...read more.

Conclusion

The other difficult variable to maintain was the temperature of the heated water. With only basic equipment, keeping the water at the correct temperature was made a complicated task, besides the effect of temperature between -5 �c to 24�c were not carried out hence the graph is not in great deal of accuracy. There are also some percentage error in the equipments that are used, such as measuring cylinder, which it is an error with 0.1cm. A pipette should be replace in order to achieve more accurate results. External variables were well controlled. If the experiment was to be repeated, the use of a proper controlled water bath may be a consideration, and also a template made for cutting the beetroot pieces. Using a beetroot as the sample is not a good representation of the whole eukaryote group. Other cell membranes may have better or worse heat tolerance, some may not be affected at all, however, using a beetroot does give a good representation of the theories behind the plasma membrane and how it behaves. For further work, we are alter the controlled and key variables of varies experiment, such as using different concentration of alcohol, since alcohol can break down lipids that are within the cell surface membrane, which will also should the effect of alcohol on membrane permeability. Jessica Leung 1 ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Green Plants as Organisms section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Green Plants as Organisms essays

  1. Marked by a teacher

    How does temperature affect the permeability of a cell membrane in a beetroot.

    4 star(s)

    I will be leaving each piece of beetroot in the water for 10 minutes I discovered this to be an effective time from my preliminary work. Treatment of beetroot The way that the beetroot is treated after being removed from the cork borer will affect the pigment released from the beetroot.

  2. Peer reviewed

    Effect of Temperature on Beetroot Membrane Proteins

    4 star(s)

    As the temperature increases, the water expands - this will have a disruptive effect on the membrane. Heat energy in the water is converted into kinetic energy in the beetroot. This would cause red pigment molecules to vibrate faster, and may have had the same effect on the phospholipids in the partially permeable cell membrane.

  1. Experiment to determine the effect of temperature on the permeability of a cell membrane ...

    if the amount was higher than others the concentration would have been lower in the tubes with more water. The segments of beetroot were measured and cut accurately otherwise the larger cubes would contain more pigment meaning the concentration would be higher in tubes containing larger cubes.

  2. An investigation in to the effect of temperature on the release of pigment from ...

    I predict that as the increase of temperature is applied, there will also be an increase in the release of red pigment out of the beetroot cells. I predict this because the increase of kinetic energy will inevitably speed up the diffusion rate of the red pigment, hence more release of red pigment out of cells will occur.

  1. the effect of temperature on membrane permeability

    Heat beetroot at 20 oc, using a thermometer to measure the temperature and maintain for 2 minutes 10. When heat reaches the required temperature, remove the boiling tube from the water bath using tongs because the tube will be hot.

  2. How Temperature Effects the Movement of Pigment Through Cell Membranes

    area will remain the same due to the same cork borer being used and the length will be cut to exactly four centimetres. The beetroots are also from the same batch of beetroots, as a difference in packs or producers or possibly the country of where the beetroots are grown

  1. Investigating the effect of temperature on permeability of membranes

    Most enzymes denature at temperatures above 50 degrees Celsius. When the proteins are denatured the phospholipid layer of the cell membrane alters and has gaps in it. The red pigments probably escape through these gaps. If temperature has no effect on the permeability then the red pigments should escape at room temperature water.

  2. An Investigation into the effect of Temperature on the release of Betalain from Beetroot ...

    The Beetroot will be left covered while not being used and the only pieces to be cut from it will be the ones for the temperature, which will be measured next. This will prevent any of the beetroot drying up as if the membranes dry up they will release less Betalain.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work