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The Effect of Temperature on the activity of the Enzyme Catalyst.

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Introduction

Yr 11 - practical investigation The Effect of Temperature on the activity of the Enzyme Catalyst My investigation requires me to investigate how temperature affects the activity of the enzymes in a potato. This experiment uses the enzyme to decompose Hydrogen Peroxide H�O�. The variable that I am going to alter in this experiment is the temperature of the H�O�. I will measure the volume of gas produced, catalyse in the potato tissue reacts with the hydrogen peroxide to create water and oxygen. I will change the temperature at which the reaction takes place, but to make the test a fair one I will keep the pH level on the solution, the amount and concentration of the solution, and the area and the volume of the potato the same. I will have to take into account the concentration of Catalyse in the potato, this cannot be controlled. Catalyse is an enzyme. The purpose of an enzyme is to act as a biological catalyst which speeds up a reaction without being consumed. A catalyst works by reducing the amount of activation energy needed for the reaction to take place. The only variable that I am going to change in this experiment is the temperature of the Hydrogen Peroxide H�O�. I will be changing the temperature 6 times. ...read more.

Middle

My results can be shown in fig. 1. After analysing my result, I came to the conclusion all three times of collection was adequate for the experiment in question. Therefore I decided to use 5 minutes, as it is in the middle, and the results that were collected were more evenly spread out. Gases can be measured in 3 ways; mass lost, into a syringe or under water, my practical preliminary saw successes using the gas syringe. Therefore it is what I would use. From my research, I have found out that Enzymes may work faster if the temperature within the cell increases as the molecules travel faster as it heats up, but if the temperature increases too much, the living cell may die and they may breakdown called denaturation. The most effective time when an enzyme works is called the optimum temperature which is 36 degrees Celsius, usually, the optimum temperatures for enzymes in living cells are close to the normal cell temperature. Reactions occur when particles collide with enough energy (activation energy) as the temperature becomes higher the particles move faster around and collide to cause the chemical reaction. Enzymes are protein molecules produced by living cells. ...read more.

Conclusion

Only the correct key will fit in the lock, so only the correct substrate will react with the enzyme. If the temperature is higher than a certain point, chemical reactions cannot take place effectively or at all. Here are my results. Temperature (�C) Time (mins) Gas collected (ml) 20 5 5 30 5 11 40 5 14 60 5 10 70 5 3 80 5 1 My experiment went very well, from my results, I could see that the 'loop' pattern I was expecting in the graph is present. I think that if I were to reattempt this experiment, I would change the variables. I could vary the time of the reaction or change the amount of slices of potatoes that I use. Improvements I could make are trying to do the whole investigation on the same day therefore the conditions in which I conduct my experiment would be more suitable and fairer in terms of fair testing. I think that the results are very good. I think this because the average results showed what I said in my prediction. I said that the largest amount of gas collected would be around 36 degrees Celsius. This is satisfied in my results. In conclusion I think my experiment went relatively smoothly, my results must be quite good because I got my prediction correct. Page 1 of 4 ...read more.

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