• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

The Effect of Temperature on the activity of the Enzyme Catalyst.

Extracts from this document...

Introduction

Yr 11 - practical investigation The Effect of Temperature on the activity of the Enzyme Catalyst My investigation requires me to investigate how temperature affects the activity of the enzymes in a potato. This experiment uses the enzyme to decompose Hydrogen Peroxide H�O�. The variable that I am going to alter in this experiment is the temperature of the H�O�. I will measure the volume of gas produced, catalyse in the potato tissue reacts with the hydrogen peroxide to create water and oxygen. I will change the temperature at which the reaction takes place, but to make the test a fair one I will keep the pH level on the solution, the amount and concentration of the solution, and the area and the volume of the potato the same. I will have to take into account the concentration of Catalyse in the potato, this cannot be controlled. Catalyse is an enzyme. The purpose of an enzyme is to act as a biological catalyst which speeds up a reaction without being consumed. A catalyst works by reducing the amount of activation energy needed for the reaction to take place. The only variable that I am going to change in this experiment is the temperature of the Hydrogen Peroxide H�O�. I will be changing the temperature 6 times. ...read more.

Middle

My results can be shown in fig. 1. After analysing my result, I came to the conclusion all three times of collection was adequate for the experiment in question. Therefore I decided to use 5 minutes, as it is in the middle, and the results that were collected were more evenly spread out. Gases can be measured in 3 ways; mass lost, into a syringe or under water, my practical preliminary saw successes using the gas syringe. Therefore it is what I would use. From my research, I have found out that Enzymes may work faster if the temperature within the cell increases as the molecules travel faster as it heats up, but if the temperature increases too much, the living cell may die and they may breakdown called denaturation. The most effective time when an enzyme works is called the optimum temperature which is 36 degrees Celsius, usually, the optimum temperatures for enzymes in living cells are close to the normal cell temperature. Reactions occur when particles collide with enough energy (activation energy) as the temperature becomes higher the particles move faster around and collide to cause the chemical reaction. Enzymes are protein molecules produced by living cells. ...read more.

Conclusion

Only the correct key will fit in the lock, so only the correct substrate will react with the enzyme. If the temperature is higher than a certain point, chemical reactions cannot take place effectively or at all. Here are my results. Temperature (�C) Time (mins) Gas collected (ml) 20 5 5 30 5 11 40 5 14 60 5 10 70 5 3 80 5 1 My experiment went very well, from my results, I could see that the 'loop' pattern I was expecting in the graph is present. I think that if I were to reattempt this experiment, I would change the variables. I could vary the time of the reaction or change the amount of slices of potatoes that I use. Improvements I could make are trying to do the whole investigation on the same day therefore the conditions in which I conduct my experiment would be more suitable and fairer in terms of fair testing. I think that the results are very good. I think this because the average results showed what I said in my prediction. I said that the largest amount of gas collected would be around 36 degrees Celsius. This is satisfied in my results. In conclusion I think my experiment went relatively smoothly, my results must be quite good because I got my prediction correct. Page 1 of 4 ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Life Processes & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Life Processes & Cells essays

  1. Marked by a teacher

    Investigating the breakdown of hydrogen peroxide by the enzyme catalyse in potatoes.

    4 star(s)

    Time/s Oxygen produced at 30�C Oxygen produced at 70�C 30 17 0 60 30 0 90 36 0 120 39 0 150 42 0 180 43 0 210 44 0 240 44 0 270 45 0 300 46 0 This is a table of our results when we were using temperature as a variable.

  2. Experiment to investigate the effect of Temperature on the enzyme activity of Pectinase

    Therefore pectinase enzymes are commonly used in processes involving the degradation of plant materials, such as speeding up the extraction of fruit juice from fruit, including apples. Pectinases have also been used in wine production since the 1960s.it joins together with the substrate at the active site Like all enzymes,

  1. Biology catalyst couursework

    The variable that I am going to use is concentration as this is the most practical out of the four variables. The collision theory explains how concentration affects the rate of reaction and therefore the amount of oxygen produced: For a reaction to occur the reactant particles must collide.

  2. How temperature affects enzyme activity?

    present, this is because they break up the substrate into smaller droplets, this means that there is an increase in surface area, making the enzymes work faster, an increase in concentration also does this because there are more enzymes to work on the substrate.

  1. The Effect of Temperature on Enzyme Activity.

    because then the length of the experiments is controlled. If the first method is used (timing how long it took to produce an X volume of oxygen gas), the time could be anywhere between 10 minutes and 3 hours! Because the investigation is centred on temperature, the temperature of the environment in which the reaction is taking place has to be varied.

  2. Find the effect of temperature on enzymes, using a potato as a catalyst - ...

    Still others release energy to make the heart beat and the lungs expand and contract. Many facilitate the conversion of sugar and foods into the various substances the body requires for tissue-building, the replacement of blood cells, and the release of chemical energy to move muscles.

  1. The effect of substrate concentration on the activity of catalyse.

    Fig 4.1: The graph produced when the activation energy is lowered. (Williams, 2000: 67) Variable Graph Why It Must Be Controlled? How Will It Be Controlled? pH A change in pH would result in the tertiary structure of catalyst changing.

  2. Structures and functions in living organisms. Revision Notes

    of smoking in relation to the lungs and the circulatory system It could cause bronchitis because the tar sticks to the cilia in the windpipe and bronchi. It could cause emphysema, reducing the surface area of alveoli. It could cause cancer.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work