• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

The effect of temperature on the rate of an enzyme catalysed reaction

Extracts from this document...

Introduction

The effect of temperature on the rate of an enzyme catalysed reaction 2H2O2 � O2+H2O Catalase Apparatus Bench mat Goggles Conical Flask Graduated pipette Bung and delivery tube Syringe 10 cm3 Hydrogen Peroxide Boss and clamp Stand Gas syringe Ice Kettle Potato Borer Stopwatch 50 ml beaker 2*250ml beaker Thermometer We are looking at the effect of catalase (in potatoes) on Hydrogen Peroxide to find the effect of temperature on the rate of an enzyme-controlled reaction. Oxygen gas will be produced therefore we can measure the volume of oxygen produced, and compare the rate which is produced at different temperatures to see the effect that changing temperature has on the rate of reaction. I will obtain my results by measuring oxygen evolution in cm3 over various temperatures. If graphs are produced we can measure the gradients of the graphs and we will obtain values for the rates of reaction, which can then be plotted on a separate graph. ...read more.

Middle

More enzyme substrate complexes are formed per second, with a corresponding increase in the rate of catalysis and on the number of product molecules. Therefore the temperature of the environment surrounding the system should be kept constant and the temperatures variables should be accurate. We already know that concentration affects enzyme activity as when concentration is increased there are more active sites available for reaction so collisions occur more frequently between substrate and enzyme, so there are more substrate complexes which form per second- so the rate of reaction increases. If enzyme concentration is varied then this will affect the rate of reaction. Can attempt to control this by using the same potato for each experiment. Surface are should be kept constant. If the piece of potato is for example cut into smaller pieces whilst another is left in a larger piece it will have a greater surface area If the potato has a larger surface area then there will be a much larger area upon which the collisions between substrate and enzymes occur. ...read more.

Conclusion

Check with a thermometer. Put the conical flask in one beaker and the smaller beaker into the other. Make sure they do not sink. Leave them to adjust to the temperature and constantly check the temperature to make sure it is right. * Using the graduated pipette, 10cm3 of Hydrogen Peroxide should be obtained and placed into the syringe. * Check that the gas syringe is set at 0 * Apply pressure to the syringe containing the Hydrogen Peroxide until all the contents have been added to the conical flask. * Immediately start the stopwatch. Take readings of oxygen evolution every 20 seconds and record them in a table. After 300 seconds stop recording results. * Wash thoroughly the entire system and repeat the above steps using the different temperatures of 20, 30 40 50 and 60oC. For the higher temperature a heating system should be used instead of a cooling system. * Ideally three sets of results should be obtained for each temperature Evaluation I conclude that generally (I have no time left to finish but will complete at earliest available opportunity) ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Life Processes & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Life Processes & Cells essays

  1. The effect of temperature upon the enzyme catalase

    The kinetic theory states that when substances are heated they gain more energy and begin to vibrate faster. Therefore when the enzyme and substrate are heated up they begin to collide with each other more frequently and the rate of successful collisions increases.

  2. My hypothesis is that the higher the concentration of hydrogen peroxide the more catalase ...

    > Then I set up a boss, stand and clamp on the table. I filled a 50ml burette with water using a funnel and overturned it inside a bowl filled with water. One end of a delivery tube was fed through and the burette was held in place with a clamp.

  1. To examine the effect of Substrate Concentration (Hydrogen Peroxide) on the rate of an ...

    These measurements are necessary to ensure that surface area and volume are kept constant. It will also be weighed to obtain maximum accuracy using a top pan balance correct to 2 d.p. This will be noted in a table because this is the fairest way to ensure that each time

  2. Investigating an enzyme-catalyzed reaction

    So I to get round this problem I would use the yeast solution mixed with distilled water so the yeast wouldn't have got stuck at the top. The next results that I got were more reliable and the experiment was easier to run and I got better results.

  1. Science and Evolution

    Science makes more sense theoretically then a religious belief and science can tell us everything about evolution. With science there is usually scientific proof that explains all most of the theory. Through the study of evolution we have made many significant discoveries.

  2. Investigating the Effect of Enzyme Concentration on Rate of Reaction.

    I decided not to count the bubbles evolved either, because firstly there were too many coming off too fast for accurate counting and I could actually see a difference in size in the bubbles. This would certainly lead to imprecise and unreliable results.

  1. An Investigation into the Effect of Temperature on an Enzyme Catalysed Reaction.

    Each subunit contains a heme group. This heme group is responsible for carrying out catalase's activity. Catalase functions to break down hydrogen peroxide (H2O2) into water and oxygen: 2H2O2 2H2O + O2 This reaction is performed by oxidation, the loss of electrons, and reduction, which is the gain of electrons.

  2. An investigation into how enzyme concentration (catalyse in a potato) affects the rate of ...

    This approach to reactions is called collision theory. From Collins study and revision science GCSE Factors that effect the rate of reaction are: * Substrate concentration * Enzyme concentration * Temperature * Pressure * Light * A Catalyst Substrate concentration A higher substrate concentration means there is a greater number of substrate molecules.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work