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The effect of temperature on the rate of an enzyme catalysed reaction

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Introduction

The effect of temperature on the rate of an enzyme catalysed reaction 2H2O2 � O2+H2O Catalase Apparatus Bench mat Goggles Conical Flask Graduated pipette Bung and delivery tube Syringe 10 cm3 Hydrogen Peroxide Boss and clamp Stand Gas syringe Ice Kettle Potato Borer Stopwatch 50 ml beaker 2*250ml beaker Thermometer We are looking at the effect of catalase (in potatoes) on Hydrogen Peroxide to find the effect of temperature on the rate of an enzyme-controlled reaction. Oxygen gas will be produced therefore we can measure the volume of oxygen produced, and compare the rate which is produced at different temperatures to see the effect that changing temperature has on the rate of reaction. I will obtain my results by measuring oxygen evolution in cm3 over various temperatures. If graphs are produced we can measure the gradients of the graphs and we will obtain values for the rates of reaction, which can then be plotted on a separate graph. ...read more.

Middle

More enzyme substrate complexes are formed per second, with a corresponding increase in the rate of catalysis and on the number of product molecules. Therefore the temperature of the environment surrounding the system should be kept constant and the temperatures variables should be accurate. We already know that concentration affects enzyme activity as when concentration is increased there are more active sites available for reaction so collisions occur more frequently between substrate and enzyme, so there are more substrate complexes which form per second- so the rate of reaction increases. If enzyme concentration is varied then this will affect the rate of reaction. Can attempt to control this by using the same potato for each experiment. Surface are should be kept constant. If the piece of potato is for example cut into smaller pieces whilst another is left in a larger piece it will have a greater surface area If the potato has a larger surface area then there will be a much larger area upon which the collisions between substrate and enzymes occur. ...read more.

Conclusion

Check with a thermometer. Put the conical flask in one beaker and the smaller beaker into the other. Make sure they do not sink. Leave them to adjust to the temperature and constantly check the temperature to make sure it is right. * Using the graduated pipette, 10cm3 of Hydrogen Peroxide should be obtained and placed into the syringe. * Check that the gas syringe is set at 0 * Apply pressure to the syringe containing the Hydrogen Peroxide until all the contents have been added to the conical flask. * Immediately start the stopwatch. Take readings of oxygen evolution every 20 seconds and record them in a table. After 300 seconds stop recording results. * Wash thoroughly the entire system and repeat the above steps using the different temperatures of 20, 30 40 50 and 60oC. For the higher temperature a heating system should be used instead of a cooling system. * Ideally three sets of results should be obtained for each temperature Evaluation I conclude that generally (I have no time left to finish but will complete at earliest available opportunity) ...read more.

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