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The effect of temperature on the reaction between amylase and starch

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Introduction

Biology Coursework The effect of temperature on the reaction between amylase and starch By Arnita Manandhar Form 11S Mrs Sharma My aim is to find out if temperature affects the rate of reaction between amylase and starch. The basic idea of my prediction was that the higher the temperature was, the faster the rate of reaction between amylase and starch. This is because heat energy causes more collisions between the particles in both the substrate and enzyme. However after remembering my past studies regarding enzymes, I realised that by prediction had to be interpreted to a certain extent due to the denaturing of enzymes in high temperatures. The Optimum temperature is the maximum temperature an enzyme can catalyse a reaction and which has the right amount of energy to reach the activation energy which begins the process. Activation energy is the energy that connects the enzyme with its particular substrate. Looking at the graph above, it is fair to say that since enzymes are used in our own bodies it is proven that after around 40�C the enzymes start to denature. Hence, this explains the theory that when we have high temperatures and are ill, the enzymes in our body do not function properly. After this comprehension, my prediction developed into: I predict that the higher the temperature, the faster the rate of reaction between amylase and starch, however after 40�C/50�C, the reaction time will slow down and the reaction will stop to take place after a certain temperature. ...read more.

Middle

This then could have decreased the temperature to the optimum temperature allowing a quick reaction to take place. To see if the results of the rate of reaction graph are accurate, I performed a Q10 experiment. Q10 is a theory that states that if you increase the temperature you are working with by 10�C, the rate of reaction should double. I have included my working out below as to whether Q10 supports the experiment or not. I would also like to add that Q10 works till the optimum temperature, thus in this case till 60�C. I have put the rate of reaction times to four decimal places to be more accurate. 1) 30�C's Rate of Reaction --> 0.0042 = 2.1 20�C's Rate of Reaction 0.0020 2) 40�C's Rate of Reaction --> 0.0094 = 2.2 (1dp) 30�C's Rate of Reaction 0.0042 3) 50�C's Rate of Reaction --> 0.0161 = 1.7 (1dp) 40�C's Rate of Reaction 0.0094 4) 60�C's Rate of Reaction --> 0.0211 = 1.3 (1dp) 50�C's Rate of Reaction 0.0161 As you can see from my analysis of Q10 went considerably well. The different rates of reactions were divides by one another to see if the answer would come to near about two. If this happened the Q10 theory would have proved itself and I right. ...read more.

Conclusion

There are a few limitations I realised after the experiment. If I were to do the experiment once again, I would carry out all the improvements I have mentioned. I would also do the experimentation at every 5�C interval to have more results which may have given me further insight on my findings. This is also a good idea to carry out, as to be fairly honest, I have no idea what happens between the intervals of 0-20, 20-30, 30-40 etc. In redoing the experimentation, I would also carry on the test the results after 90�C as I am unaware if anything could have changed to maybe prove my prediction wrong. Further work, which I could possibly carry out to add to this investigation, is to carry out another experiment on the affect of temperature between another enzyme and substrate, like carbohydrase and carbohydrates. From this I can compare the results I get from that experimentation to the one with amylase and starch to configure whether there is any relationship with their trends and optimum temperatures. This could help enable the further reliability of my initial investigation. Overall, I am proud to say that the investigation has followed out to be a successful experimentation. Besides the anomalous results, the rest of the results are quite close to each other fitting a trend which proves the experiment to be fairly reliable. I have productively managed to establish my prediction and provide evidence from scientific knowledge as well as my own. ...read more.

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