Apparatus: The apparatus that I will need are going to be as follows:
- Yeast
- Glucose
- Measuring cylinder
- Thermometer
- Water Bottles
- Stop clock
- Balance
- Boiling tube
- Delivery tube
Method:
1. Collect the equipment.
2. Set up a water bath at the temperature of 20 degrees C.
As I have to explore the effect of temperature on yeast, I decided that I will carry out the experiment with 5 different temperatures, 0, 20, 40, 60, and 80 degrees C.
3. Place a beaker full of water inside the water bath so that the beaker reaches the temperature of the bath (20 degrees C).
4. Measure 0.9 gm of glucose. According to my preliminary experiment, I believe this is the appropriate amount to use for this experiment as it is not too excessive and it’s not too little either. Also, I found it out to be the optimum amount.
5. I will place the glucose and yeast in the same boiling tube and add water to it.
6. I will also put in a suitable amount of water into it, not too much, but not too little.
7. As the reaction starts, I will put on end of the delivery tube (the end with the cork) into the boiling tube, ad the other end into the measuring cylinder, almost full of water, which I have already put into the water bath.
8. Now, as the reaction takes place, the gas which has been produced inside the boiling tube will move, via the delivery tube, into the cylinder. And as more gas is produced, the more the water inside the cylinder will be displaced, meaning that I can measure, roughly, the amount of gas collected by jus looking at the measurements on the cylinder. I will keep the experiment going on for 5-10 minutes, as to give the reaction time to react, but not too much time.
Fair Testing: To keep the test fair, I will keep the level of water in the bath, cylinder, and boiling tube the same. I will also keep the amount of glucose and yeast the same, as this will be fairer.
Hypothesis: I predict that the temperature that the enzyme will work best at 40 degrees C as this is the closest temperature to our body, and enzymes work in our body. This is the optimum temperature for the enzymes to work well at. Other temperatures will not work best because if the temperature is too high (80 degrees C), or if it is too low (0 degrees C) then the enzymes will denature because the heat is likely to kill them.
