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The effect of temperature upon the enzyme catalase

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The Effect of Temperature upon the Enzyme Catalase Enzymes are biological catalysts; they speed up chemical reactions within living organisms. These reactions can only take place under a certain environment where many factors will affect the reaction; one of these factors is temperature. The aim of this experiment is to determine the effects of temperature upon the enzyme catalase. I looked at the amount of oxygen was given off at different temperatures when the enzyme catalase was reacted with hydrogen peroxide. The reaction's equation is as follows:- To understand the effect temperature has upon the enzyme catalase, the experiment will only be affected by the temperature. Any other factors will be kept constant. The temperatures I measured were; 0oC, 20oC, 40oC, 60oC, and finally 80oC. Independent variable- The temperature of the catalase Dependant variable- The volume of oxygen collected every 2 minutes Variables kept constant:- * The amount of hydrogen peroxide (5cm3) * Amount of catalase (5 grams) * Surface area of the catalse * pH * Time at which the volume of Oxygen is collected Apparatus Syringe Stopper with two holes Test Tube Temperature controlled Water Tank Water bath Container Filled with water Delivery Tube 100 cm3 Measuring Cylinder 5 grams of potato (Catalase source) 5cm3 Hydrogen peroxide Stop watch Knife Ruler Chopping Board Pencil and Paper Ice Weighing scale (2 d.p) Potato Peeler Method A potato peeler will be used to peel off the skin of a potato. ...read more.


Therefore we can discover a general trend from this graph; as the temperature increases so does the amount of oxygen given off, until the enzyme reaches its optimum temperature whereby the reaction begins to slow down and produce less oxygen as the temperature rises, until the reaction is finally no longer able to take place. These conclusions can be explained if we look at graph 2, the graph with the mean points plotted on it. As we can see this graph also follows the same general trend as the graph with all the points plotted. Part A of the graph is the results from 0oC-40oC. In this range we can see that the amount of oxygen given off is increasing as the temperature rises, this is because as the temperature rises the molecules will have more energy and therefore there will be more successful collisions faster and more frequently between the enzyme and its substrate, thus more oxygen will be released. Part B of the graph shows the optimum temperature of the enzyme. This is where the enzyme reaches its maximum rate of successful collisions. There is no way of telling what the optimum temperature actually was. We know that the optimum temperature must lie between 23.4oC and 60oC however there is no way of pinpointing the temperature where the most successful collisions occurred. However from looking at my graph it is most likely that the optimum temperature lies between 30oC and 50oC. ...read more.


0 23 40 60 74 Volume of oxygen collected within 2 minutes (cm3) Group 1 15 22 30 11 4 Group 2 12 29 46 21 21 Group 3 10 28 32 11 5 Group 4 16 43 51 45 46 Group 5 11 31 42 7 5 Group 6 14 28 41 15 0 Group 7 10 27 38 20 5 Group 8 13 34 47 10 5 Group 9 21 24 34 19 3 Group 10 11 28 36 21 5 MEAN VOLUME (not including outliers) 12.4 27.8 38.4 15 4.57 From looking at the different temperatures I measured we can see that there were irregular class intervals. This is because the temperature of 23oC was supposed to be 20oC however the room temperature on that day was 23oC. The temperature of 74oC was also supposed to be 80oC, however the water bath we used was not able to reach 80oC and the maximum it could sustain was 74. This would not largely affect the analysis of the graph however the inconsistent intervals may limit the safety of any conclusions drawn. We can see from this table of results that there is a very wide range of data; the 40oC class had a very large range of 17cm3, thus showing large levels of inaccuracy. The data also has a lot of outliers as visible (shaded results), showing that the experiment must have had some major flaws in it to give such inaccurate results. These vast amounts of outliers meant that I only had 43 useful results, also hindering my ability to make accurate conclusions. ?? ?? ?? ?? Sahil Patel ...read more.

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