• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

The Effect Of Varying Enzyme Concentration On The Breakdown Of Hydrogen Peroxide In The Presence Of Catalase

Extracts from this document...


The Effect Of Varying Enzyme Concentration On The Breakdown Of Hydrogen Peroxide In The Presence Of Catalase Aim This is an experiment to find out how the concentration of the of the enzyme catalase affects the rate of reaction of the substrate Hydrogen Peroxide. Intro Enzymes like catalase are protein molecules that are found in living cells. They are used to speed up reactions within the cell. Catalase is found in food such as potato that I will be using in my experiment. They are all very specific as each enzyme is used for different jobs and Catalase is used for removing Hydrogen Peroxide from the cells. ...read more.


Apparatus Two 10cm3 measuring cylinder Hydrogen Peroxide (1M) Water bath A potato Delivery tube Bung Conical flask A razor blade A stopwatch A ruler Cork borer Method 1. First of all I connected the delivery tube to the conical flask then I filled a tub full of water and placed the delivery tube in the water. 2. Then I measured out the 1M of Hydrogen peroxide and placed this into the conical flask. 3. Then I used a cork borer to get a long piece of potato out of the potato. I cut this up first into 1cm then 2cm, 3cm and 4cm. I also measured each of these after that to find the surface area for each. ...read more.


This is because the higher the enzyme concentration so the bigger the potato the more active sites there are. So there for there will be more successful collisions take place between the enzyme and the substrate. Evaluation My experiment worked quite well in the end but if I were to do it again I would change a few things to make my results more accurate. Firstly I would do the get more readings by using more potato instead of stopping at 4cm because I hadn't reached the optimum rate of catalase activity. Also I would use the same amount of potato twice to see if there really is the same amount of catalase in each cell. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Life Processes & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Life Processes & Cells essays

  1. Investagating the Action of the Enzyme Catalase On the Surface Area of a Potato.

    the same distance each time and using the same amount and concentration of the hydrogen peroxide. I also made sure that I judged how much oxygen was collected fairly for every experiment and I always timed each experiment up to 10 minutes.

  2. Investigating the Effect of Substrate Concentration on Catalase

    Concentration table: Concentration/ % Volume of H2O2/cm3 Volume of Distilled Water/cm3 0.0 0 12 0.5 2 10 1.0 4 8 1.5 6 6 2.0 8 4 2.5 10 2 3.0 12 0 This concentration table shows how much of each substance will be needed in order to make up the different concentrations.

  1. Investigating the effect of enzyme catalase concentration on hydrogen peroxide.

    The temperature of the experiment was important but to a certain extent we could not keep room temperature constant but it was checked with a thermometer. 5) The time was checked constantly by a stopwatch so that this time counting would help us to work out the rate of bubble release.

  2. Investigate the effect of the concentration of the enzyme catalase on the decomposition of ...

    It will have to cover the entire surface of the potato. I will also need to find out what the best way of cutting the potato is and how long I will need to time the experiment for to gain a wide range of results because I do not know how fast the reaction is.

  1. The effect of hydrogen peroxide on catalase if you change the temperature.

    This is in line with kinetic theory. However it is very clear that after a certain temperature is reached the enzyme actually virtually stops. This supports my theory of lock and key fit. However optimum activity of enzyme is at about 37�C this is as we expected.

  2. The Effect Of Changing The Surface Area Of Potato On The Breakdown Of Hydrogen ...

    With different volumes, we would have an unfair Experiment. Time The time to let the reaction take place has to be constant because if there was more time given in one of the tests, then more oxygen will be produced because more successful collisions will take place, since there is more time available to let the reactions take place.

  1. The Decomposition of Hydrogen Peroxide.

    of the hydrogen peroxide, the volume of hydrogen peroxide that is to be decomposed, the amount of light the experiment is exposed to, and the concentration of the hydrogen peroxide. The mass of the potato will alter how much of the enzyme catalase is available to catalyse the decomposition and

  2. The aim of this experiment is to demonstrate that the substrate Hydrogen Peroxide will ...

    reaction and they are listed below, with an explanation of how and why they will affect the reaction, and how they will be controlled/varied. Temperature: As temperature increases, molecules move faster (Kinetic Theory), thus increasing the chances of collision of the substrate with the enzyme's active site.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work