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The Effect Of Varying Enzyme Concentration On The Breakdown Of Hydrogen Peroxide In The Presence Of Catalase

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Introduction

The Effect Of Varying Enzyme Concentration On The Breakdown Of Hydrogen Peroxide In The Presence Of Catalase Aim This is an experiment to find out how the concentration of the of the enzyme catalase affects the rate of reaction of the substrate Hydrogen Peroxide. Intro Enzymes like catalase are protein molecules that are found in living cells. They are used to speed up reactions within the cell. Catalase is found in food such as potato that I will be using in my experiment. They are all very specific as each enzyme is used for different jobs and Catalase is used for removing Hydrogen Peroxide from the cells. ...read more.

Middle

Apparatus Two 10cm3 measuring cylinder Hydrogen Peroxide (1M) Water bath A potato Delivery tube Bung Conical flask A razor blade A stopwatch A ruler Cork borer Method 1. First of all I connected the delivery tube to the conical flask then I filled a tub full of water and placed the delivery tube in the water. 2. Then I measured out the 1M of Hydrogen peroxide and placed this into the conical flask. 3. Then I used a cork borer to get a long piece of potato out of the potato. I cut this up first into 1cm then 2cm, 3cm and 4cm. I also measured each of these after that to find the surface area for each. ...read more.

Conclusion

This is because the higher the enzyme concentration so the bigger the potato the more active sites there are. So there for there will be more successful collisions take place between the enzyme and the substrate. Evaluation My experiment worked quite well in the end but if I were to do it again I would change a few things to make my results more accurate. Firstly I would do the get more readings by using more potato instead of stopping at 4cm because I hadn't reached the optimum rate of catalase activity. Also I would use the same amount of potato twice to see if there really is the same amount of catalase in each cell. ...read more.

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