• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

The Effects of pH on the Function of Catalase.

Extracts from this document...


Amanda Rosen November 5, 2002 Biology The Effects of pH on the Function of Catalase Introduction: Hydrogen peroxide is a toxic compound produced by living organisms. Under normal conditions these organisms also produce an enzyme that quickly changes hydrogen peroxide into two harmless substances, oxygen and water. However, the function of the enzyme is affected by changes in the environment. Our knowledge of the decomposition of hydrogen peroxide and the function of enzymes is leading to a variety of applications, from treating influenza patients to testing for properly cooked poultry. Purpose: What is the effect of altering the pH on the function of catalase? Hypothesis: When altering the pH of a catalase it will speed up the reaction rate of the catalase. ...read more.


9. Cover the potato slices in cup 5 with soda. 10. Determine the pH of the solution in each cup by holding a piece of pH paper with forceps and dipping it into the liquid. Use a new piece of pH paper for each solution. Record the pH in Table 1. 11.Measure exactly 5 ml of hydrogen peroxide in a graduated cylinder and add it to each container. 12.Observe each container for three minutes before you record your observations in Table 1. You are making qualitative measurements for this activity. Positive (+) indicates that a reaction is observed; negative (-) means that a reaction is not observed. Observations: Table 1: Results of a study of the effects of pH on enzyme behavior. ...read more.


The tests at the extreme pH values (acetic acid and sodium hydroxide) produced negative results while the other tests were positive. Application of principles Conclusions: 1. State the pH range in which catalase can decompose hydrogen peroxide. The damaged cells around the wound release catalase (or some similar enzyme). The unbroken skin around the wound does not release catalase. 2. What environmental factors affect the rate at which an enzyme functions? Since catalase is present at the site of a skin wound, hydrogen peroxide is being decomposed rapidly. Surfaces do not have enzymes and so the hydrogen peroxide lasts longer and can more effectively kill any bacteria in the area. 3. How does the protein portion of an enzyme affect the behavior of the enzyme? Enzyme action is affected by the pH of the environment. The result of the acetic acid test shows that a low pH prevents an enzyme from functioning. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Life Processes & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Life Processes & Cells essays

  1. Marked by a teacher

    The Effect of pH on Catalase

    4 star(s)

    5 cm3 with 10 cm3 distilled water 2. 1 cm3 with no distilled water Time (s) Volume of O2 produced (cm3 ) 1. 15 vol. H2O2 2. 20 vol. H2O2 10 4 3 20 5 5 30 6 6 40 7 8 50 8 9 60 9 11 120 13

  2. Investagating the Action of the Enzyme Catalase On the Surface Area of a Potato.

    tube was always the same because if a tube is thicker, it can lead to more oxygen being collected because the thicker tube allows more oxygen to pass through. Therefore, to make it fair I always made sure that I kept the same tube.

  1. What influence does pH have on the enzyme Catalase?

    Moreover, they show a trend that as the pH buffer was increased; more oxygen was made and evolved the manometer liquid showing that rate of reaction between catalase and hydrogen peroxide increased. Figure 1 represents this with the positive correlation of the curve. The values also concur with the trend.

  2. Investigating the influence of pH on the activity of potato catalase

    Therefore, breaking these bonds means that the shape of the substrate, hydrogen peroxide is no longer complementary to the altered shape of the active site on the enzyme. As the hydrogen peroxide molecules no longer fit in the active site, no more potato catalase and hydrogen peroxide molecules can combine

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work