The Effects of pH on the Function of Catalase.

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Amanda Rosen

November 5, 2002

Biology 

The Effects of pH on the Function of Catalase

Introduction: Hydrogen peroxide is a toxic compound produced by living

organisms. Under normal conditions these organisms also produce an enzyme that quickly changes hydrogen peroxide into two harmless substances, oxygen and water. However, the function of the enzyme is affected by changes in the environment. Our knowledge of the decomposition of hydrogen peroxide and the function of enzymes is leading to a variety of applications, from treating influenza patients to testing for properly cooked poultry.

Purpose: What is the effect of altering the pH on the function of catalase?

Hypothesis: When altering the pH of a catalase it will speed up the reaction rate of the catalase.  

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Materials:

Procedure:

1. Cut four cubes of potato approximately 1 cm on each side. Remove the skin from each cube.

2. Cut each cube into four slices.

3. Number small plastic cups 1, 2, 3, and 4 using a permanent marker.

4. Place the four slices of potato into each of the four cups.

5. Cover the potato slices in cup 1 with water.

6. Cover the potato slices in cup 2 with vinegar.

7. Cover the potato slices in cup 3 with 0.1 M NaOH.

8. Cover the potato slices in cup 4 with detergent.

9. Cover ...

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