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The effects of temperature on the action of the enzymes catalyse.

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Introduction

Science Coursework Title: The effects of temperature on the action of the enzymes catalyse. Aim: To see how changing the temperature affects the action of the enzyme. MY HYPOTHESIS: I think that as the temperature increase the quicker the reaction will become Outline of method You add ice to 150cm� of cold water in a beaker to bring its temperature down to 10c�. Then a fixed volume of hydrogen peroxide is added to a pre-weighed piece of liver contained in a test tube. Then the maximum height reached by the foam produce was tabulated. For temperatures that were needed to be higher then room temperature, boiling water was heated in a kettle then added to reach the desired temperature. The procedure was repeated at the following additional temperatures: 10c�, 20�C, 30�C, 40�C, 50�C, 60�C and 70�C. Key variables 1.The independent variable, the thing that you change. 2.The dependent variable, the thing that changes because you have changed independent variable. 3.They control the thing(s) that you keep the same to make a fair test. Explained in detail The thing that I change is the temperature. The dependent variable should be the height of the foam. The controls are the same volume of peroxide, the same concentration is the catalyse and of hydrogen peroxide and the same mass of liver. Catalyst explained Catalyst is a substance that increases the rate of a chemical reaction. ...read more.

Middle

Some colliding particles just bounce off each other. As the temperature soars the particles are moving fast this makes particles collide more frequently in a certain time and makes it likely that collisions result in a reaction. Increasing the temperature of a reaction increases the rate of a reaction. At the higher temperatures reactant particles move more rapidly and collide more frequently and more aggressively. How each part of the apparatus did I use? Ice: To lower the waters temperature. Measuring cylinder: To measure volume of hydrogen peroxide. Thermometer: To determine the temperatures. Kettle: To raise the waters temperature. Ruler (30cm): To make measurements of the height of the foam. Liver: To provide us the enzymes. Test tubes: to hold the liver in. Beaker: to accommodate the water in. Hydrogen peroxide: to combine with the enzyme liver. Results of Investigation Test tube Volume of hydrogen peroxide (h�o�) (cm�) Temperature (�C) Height of foam (CM�) 1 Height of foam (CM�) 2 Height of foam (CM�) 3 Height of foam (CM�) 4 Height of foam (CM�) 5 Average of height of foam A 5 10� 9.3 8.2 7.2 4.5 9.4 7.9 B 5 20� 2.0 7.2 6.4 5.9 12.9 6.88 C 5 30� 4.5 5.2 3.9 4.8 9.2 5.52 D 5 40� 18 9.2 5.9 6.2 7.9 9.44 E 5 50� 12.5 9.2 4.5 6.9 7.1 8.04 F 5 60� 9.5 10.9 11.2 10.1 9.1 10.16 G 5 70� 4.9 5.1 12.5 15.9 16.7 11.02 Conclusion and Interpretations I expected these results because as the temperature is increases the height of the foam goes up. ...read more.

Conclusion

Another problem was that if the foam went higher than the test tube I would not be able to give an accurate reading. I think that if I were to do this experiment again the ways in which I could improve it are as follows; attractive looking graphs, a much more thorough description of things and more time, measure the liver twice to give a accurate amount each time and clean the test tubes after using them and do the experiment more times to give us a better and reliable result. The things that didn't go according to plan were my results of the experiment were not fair, so therefore I had to do the experiment again, this limited my time. By the time I had redone the experiment again and collected the results for a second time I had less time to finalize my coursework to hand it in on the deadline date given to me. Problems that I had were making an exact measurement on the height of the foam. As the foam bubbles was not all level. I resolved this problem by measuring the foam where most bubbles were instead of a couple on its own. If I had more time I would do the experiments more times to give and better synopsis and do more graphs to represent the data. ?? ?? ?? ?? 1 ...read more.

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