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The Rate of Bubbling of Elodea.

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Biology Investigation: The Rate of Bubbling of Elodea Prediction I predict that as the temperature is increased the rate of bubbling of elodea will accelerate then drop off after a certain temperature until it stops bubbling. I predict this because as the temperature rises the elodea will gain more energy and will be able to photosynthesise quicker, causing more bubbles. This will occur because chlorophyll is an enzyme and thus requires energy to work but also has an optimum range for temperature. Chlorophyll works rather like a lock and key. It is important that it are a very specific shape for their purpose (in this case chlorophyll joins carbon dioxide and water together to form glucose). If a key is heated too much, it melts and becomes denatured. It will no longer fit the lock it was designed for. This is why enzymes start to work less well at high temperatures. I think that at the lower temperatures (i.e. 0-20�C) we will not see many bubbles being produced. This is because enzymes need energy to work, which they get form heat and light. So when the temperature is quite low the chlorophyll does not have much energy, therefore the rate of photosynthesis will be low. As the temperature increases, I predict that the rate of photosynthesis will increase and more bubbles will be produced. This is because more heat energy is being provided to the chlorophyll, meaning that photosynthesis can happen more quickly. ...read more.


I stood up throughout the experiment due to dealing with warm and sometimes boiling water. Apparatus Results Average number of bubbles per minute Tube A B C D E F Temp. (oC) 0 1 0 0 1 0 0 10 35 23 4 30 31 38 20 70 52 10 64 63 72 30 100 71 15 97 12 107 40 85 55 9 59 2 70 50 68 23 3 25 0 32 60 5 3 0 8 0 3 70 1 0 0 2 0 0 80 0 14 0 0 0 0 Conclusion The graph obtained supports the prediction I made. It shows that the number of bubbles produced and therefore the rate of photosynthesis increased rapidly until around the 30oC where it drops steadily and stops producing bubbles completely in nearly all cases by 80oC. The anomalous result, shown in italics, is anomalous because it is so contrasting to all other results of the same temperature and doesn't fit into its own line at all. I conclude that the temperatures effect on photosynthesis is important and that the optimum temperature for elodea photosynthesis is around 30 oC. At the lower and higher temperatures the plant was unable to produce many bubbles, if any. Chlorophyll is used to make photosynthesis happen in plants. It is an enzyme, and all enzymes need some heat to activate them. ...read more.


This way we would catch the pondweed at times when it was working at a high level and at times when it was working at a low level. Another thing that was not very suitable was the way of measuring the rate of reaction. Counting the bubbles got very boring and I could easily have miscounted, leading to anomalous results. A better way would be to find some way of collecting the oxygen bubbles in a capillary tube and measuring how far along the oxygen goes. We would have to fill the capillary tube with water and seal the top, and attach the tube to the pondweed. There were no anomalous results, but if there were there are many reasons why it could be. I could have miscounted the bubbles. Also, the pondweed might not have been bubbling properly due to a deficiency in quality that I did not notice. Similarly, there might not have been enough sodium hydrogen carbonate solution, which provides carbon dioxide for the plant. Also, there is the possibility that the piece of pondweed used was damaged, for example perhaps it had been heated up too much and the chlorophyll had become denatured. The experiment worked but the wide range in rate of reaction shows how the experiment was highly unreliable. To stop this unreliability experiments would have to be carried out in the same spot at the same time of day and with the same apparatus every time. The experiment does show, however, that the rate of photosynthesis is directly affected by temperature. ...read more.

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