The aim of the investigation was to determine the quality of the water that contains chemical dye pollution. To begin the investigation firstly we put a line on the tine layer paper and carefully a spot on the stationary phase. In to the beaker mixing two different chemical, methanol and ethanol then placed the paper in to the beaker. And leave the paper about 10 minute, until the solvent move up. Finally different components of the mixture moved up at different rates.
However to identify the chemical that contain dye pollution it is important to do several test. By changing the variable and chemicals, having done the first test again we performed paper chromatography. Separations in paper chromatography involve the same principles as those in thin layer chromatography. In paper chromatography, like thin layer chromatography substances are distributed between a stationary phases. The stationary phase is usually a piece of high quality filter paper.
Having done this two test finally we interpreted the data using the Rf value for each spot.
The below table shows the Rf value and the distance each component obtained from the investigation
Rf = distance travelled by component = 4.5 0.849
Distance travelled by solvent front 5.3
Methanol and ethanol
Rf = distance travelled by component = 2.4 0.59
Distance travelled by solvent front 4.1
Water and methanol
Method 2
Determine the absorbance value for the river water at a range of wavelengths.
Result
The identity of the chemical pollutant dyes is between A and B, because the test I have done shows that the dye chemical appear blue and yellow when performing water and methanol. Also shows blue and yellow in methanol and ethanol test. Moreover when comparing the Rf value it is relatively the same, therefore the pollutant dyes chemical is A or B.
(8) According to the result obtained from the investigation the company has been broken the law, as the legal maximum point of concentration is 1% the investigation prove that the dye chemical contain 3% pollutant. For this reason I suggest criminal investigation should take place as this water can be dangerous for environment and other invertebrate that reside in the river.
