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Tine layer and paper chromatography experiment. The aim of the investigation was to determine the quality of the water that contains chemical dye pollution. To begin the investigation firstly we put a line on the tine layer paper

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Task 2 M1 1, the important of health and safety rules in workplace is to provide employees with a safe, healthy and hygienic working environment. Health and safety at work act (1974) has been made to prevent deaths and injuries at any type of workplace. Generally the important of health and safety regulation is responsibilities for your self and other people who may be affected by your action. Task 2 M3 3, the analytical techniques that carried out during this investigation was tine layer and paper chromatography. Paper chromatography and tine layer chromatography is one of the methods for testing the purity of compound and identifying substances. These two methods is a useful because it is relatively quick and requires small quantities of material. ...read more.


In paper chromatography, like thin layer chromatography substances are distributed between a stationary phases. The stationary phase is usually a piece of high quality filter paper. Having done this two test finally we interpreted the data using the Rf value for each spot. The below table shows the Rf value and the distance each component obtained from the investigation Rf = distance travelled by component = 4.5 0.849 Distance travelled by solvent front 5.3 Methanol and ethanol Spot Distance moved from baseline Rf value Blue/ yellow 4,5 / 5.3= 0.85 4.1/5.3= 0.77 Blue= 0.85/ yellow= 0.77 Yellow/ red 4.6/ 5.3= 0.87 4.7/5.3= 0.89 Yellow= 0.87/ red = 0.89 Red 4.8/ 5.3= 0.91 Red = 0.91 Blue/ red 4.4/ 5.3= 0.83 4.7/5.3= 0.89 Blue = 0.83/ red = 0.89 unknown 4.1/ 5.3= 0.77 ...read more.


0.80 0.61 0.45 690 0.21 0.11 0.83 0.67 0.51 The identity of the chemical pollutant dyes is between A and B, because the test I have done shows that the dye chemical appear blue and yellow when performing water and methanol. Also shows blue and yellow in methanol and ethanol test. Moreover when comparing the Rf value it is relatively the same, therefore the pollutant dyes chemical is A or B. (8) According to the result obtained from the investigation the company has been broken the law, as the legal maximum point of concentration is 1% the investigation prove that the dye chemical contain 3% pollutant. For this reason I suggest criminal investigation should take place as this water can be dangerous for environment and other invertebrate that reside in the river. ...read more.

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