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To discover which temperature gives the best condition for anaerobic respiration to occur in yeast.

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Introduction

The Effect of Temperature on the Rate of Anaerobic Respiration in Yeast AIM: To discover which temperature gives the best condition for anaerobic respiration to occur in yeast. EQUATION: For anaerobic respiration in yeast, the equation is: Glucose (Enzyme-yeast) Alcohol + Carbon Dioxide + Energy C6H1206 (Enzyme-yeast) 2 C2H5OH + 2 CO2 + 2ATP PREDICTION: There are several factors that could have been looked at to investigate the rate of anaerobic respiration in yeast: Concentration of solution Temperature of solution Age and size of yeast particles The chosen variable was temperature. By looking at an experiment where the effect of temperature on the enzyme Amylase was investigated, it is possible to predict that with a higher temperature, the enzyme (yeast in this case) will act faster. It acts faster as with more heat, more energy is produced, and with a greater amount of energy, the particles move faster and act more rapidly, as heat is a form of energy. In the previous experiment it was concluded that when the mixture of Amylase and Starch was heated, the Amylase broke down the Starch more quickly that it did at room temperature. By looking at that experiments conclusions, it can be predicted that the warmer the Glucose and Yeast solution is, the faster the Yeast will break down the Glucose, therefore the more rapid the production of Carbon Dioxide bubbles will be, therefore showing that the higher the temperature, the faster the rate of Anaerobic Respiration will be in Yeast. ...read more.

Middle

6. This process is repeated with the other three ice cream tubs of water at different temperatures, using the same measurement of 10ml of glucose and yeast solution with the same concentration. Again one-minute is timed and the number of bubbles is counted then recorded for each of the experiments. 7. This whole experiment should then be repeated a further two times so as to get the most accurate results possible. In order to make this experiment fair, the same volume of water will be used in each of the four ice cream tubs, the same volume of glucose and yeast solution will be used in each of the four syringes, the same concentration of glucose and yeast solution will be used in each of the syringes, the same time of one minute will be used for each of the tests, and the same method will be used for each of the four parts of the experiment. The only factor being changed will be the temperature of the water in the ice cream tub, as this is the factor I have chosen to vary. Other factors I could have also investigated would have been the concentration percentage of glucose in the yeast and glucose solution, or the amount of yeast used. It will be important to leave the experiment undisturbed during the process of respiring, as stirring, movement or air getting into the solution could alter my results. ...read more.

Conclusion

Also, as we were only counting the individual bubbles of Carbon Dioxide, it is quite possible that we miscounted, as there were frequent occasions when the bubbles were produced quite rapidly. It would have been much more fair and reliable if it had been possible to collect the bubbles of Carbon Dioxide in some form of measuring apparatus, and then measured the volume of CO2. Besides the factor that it is possible that we miscounted these bubbles, there is also the fact that some of these bubbles of Carbon Dioxide could, and were evidently larger than others, making some of our results slightly unfair and possibly unreliable. In order to provide additional relevant evidence, I think it would be required that this whole experiment were repeated three more times, and that the bubbles of Carbon Dioxide are collected in some form of apparatus. Also, due to leakage, it was necessary to fill the syringe only half full with solution, making half of it full with air (air containing part CO2). This I feel made the experiment slightly more unreliable, and I therefore would feel that if I were to repeat the experiment it would be necessary to find another form of apparatus to contain the glucose/yeast solution in. I would also like to carry out other experiments investigating the effects of other factors on the rate of anaerobic respiration in yeast, ie the concentration of the glucose/yeast solution. I would also find it interesting to continue the experiment until an optimum temperature was found, so as to make any results more accurate ...read more.

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