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To examine the effects of various physio-chemical treatments upon the permeability of beetroot cells.

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Introduction

Toyin Dawudu Biology LBio1 October 03 Aim: To examine the effects of various physio-chemical treatments upon the permeability of beetroot cells. Hypothesis: The betacyanin pigment of beet roots is normally sequestered in the vacuole and, by means of the properties of the tonoplast and cell membrane, does not leak into the cytosol or the extra-cellular sap of the beet root. Of course if the beet root is cut cells are sliced open and the pigment spills out, but if the membrane is altered (phospholipid bilayer + proteins) more subtly leakage (diffusion) of betacyanin is induced. (Reference to www.mrothery.co.uk/cells/resources/betacyanin.doc) Apparatus: Take care using a cork borer. ...read more.

Middle

Whilst actually cutting the beetroot pieces, I discovered that it was easier for me to cut out the pieces into the required length after having cut each uniform cylinder. Method: As shown in the worksheet given by Mr Cliff Results: My Results show a good progression in the trend of results, Temperature(00) Transmission (%) 5oc 94% 200c 100% 40�C 68% 60�C, 38% 80�C 9% (As Shown in the Graph) Accuracy of test: The accuracy of the experiment will be affect by certain factors such as these: 1. Error and uncertainty of the timing of the Minute e.g. 2. Uncertainty of the measuring of the Beetroot 3. ...read more.

Conclusion

By that temperature, most colours has already leaked out and therefore resulting in the highest transmission Interpretation: The overall trend seems to be that as the temperature increases, the percentage of transmission decreases expect for the exceptional anomaly which is 200c (Room Temperature). This is suggesting that membrane permeability increases with temperature expect for one exception and therefore more colour leaks out. The darker the colour means that more pigments have leaked out and therefore the lower percentage of transmission Evaluation: * The size of the borer used, will affect the amount of betacyanin in the cylindrical beetroot therefore that will affect the result. Improvements: * The * The amount of times the experiment was repeated. * The fluctuation temperature of the actual water bath could have been kept at the authentic temperature. . . ...read more.

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