• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month
  1. 1
  2. 2
  3. 3
  4. 4
  5. 5
  6. 6
  7. 7
  8. 8
  9. 9
  10. 10
  11. 11
  12. 12

To find out how enzymes are affected by a variation in Temperature, the enzyme for the study is Catalase commonly found in potatoes. I will also find the optimum temperature of when the enzyme reacts quickest.

Extracts from this document...


Aim: To find out how enzymes are affected by a variation in Temperature, the enzyme for the study is Catalase commonly found in potatoes. I will also find the optimum temperature of when the enzyme reacts quickest. Hypothesis: The hypothesis for this investigation is that when the temperature is increased, the amount of bubbles will increase in one minute so therefore the rate of reaction will increase. I believe this hypothesis to be true because of the collision theory that particles with more energy will move faster increasing the chance that a reaction will take place. Also the particles will have more energy so there will be more chance of the activation energy being reached. I also predicted that there will be an optimum temperature of around 40oC I predict this because body temperature is at about 37 oC. There will be an optimum temperature because enzymes denature at certain temperatures; denaturing is when the active site changes shape and the substrate cannot fit so it can be broken down. This is known as the lock and key theory. This causes that there is no reaction and no or very little bubbles will be counted. ...read more.


Concentration: This will be kept constant by always using the same concentration of liquids provided. pH: The pH will be kept constant by not changing the pH of the Hydrogen Peroxide and the Enzyme, Catalase. Volume of hydrogen Peroxide: This will be kept constant at 25ml by using a measuring cylinder. Apparatus: This will be kept constant by using my preliminary work r chose appropriate apparatus that will give me accurate results and using the same apparatus. Starting of Stopwatch: I have chosen to regulate the starting point of the stopwatch this will always be started at when the rubber bung with the delivery tube in it has been inserted. Apparatus and Chemicals used in experiment: 1x 25ml Measuring Cylinder Hydrogen Peroxide Water Potato 8x Test Tubes 1x Delivery Tube with bung Test Tube Rack 4x Water Baths (one at each of the following temperature 300C, 400C, 500C, 600C.) 2x thermometer 2x Cork Borers (one 8mm and the other of smaller dimension) 1x Stopwatch 1x Cutting Tile 1x Scalpel 1x Ruler Picture of Apparatus: Method: 1. I collected the apparatus, which is listed above I then, set it up as pictured above. ...read more.


I collected no anomalies in this experiment but this was due to the use of an accurate method and the processing of my results into rates of reactions and averages. This investigation could however be improved by taking a larger range of temperature at more frequent intervals this would give you also a more accurate optimum temperature and greater understanding of the process of Enzyme Action. I could not do this due to lack of time and resources. I may have collected data that could be inaccurate because when the potato and the Hydrogen peroxide are mixed, I had to push the bung and delivery tube into the top of the test tube and I had to start the stopwatch add and also count bubbles. I could have missed a bubble and also I could have started the stopwatch too late or too early and when the bung is pushed in this causes the air inside the test tube to be compressed. Therefore bubbles of air not oxygen can be produced and can be mistakenly counted as oxygen produced by the reaction. This could have been improved by having an assistant or using a n airlock system. By using these improvements in my investigation I could improve the accuracy of the results. Andrew Wright 5th Form Biology Coursework (Catalase) 1 ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Life Processes & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Life Processes & Cells essays

  1. Marked by a teacher

    Investigating the breakdown of hydrogen peroxide by the enzyme catalyse in potatoes.

    4 star(s)

    This is because they would give us a broad range of results and that we would be able to graph them and they you aid us to work out the rate of reaction. However we soon changed this to 6 as the amount of hydrogen peroxide was not that of equal to the amount of enzyme.

  2. Marked by a teacher

    Biology Coursework Enzymes

    4 star(s)

    Main Experiment Prediction By analysing my preliminary work and also using my own scientific knowledge I hypothesize that the starch concentration will definitely affect the rate of reaction and the time taken for the amylase to break down starch into products.

  1. For my coursework I will be performing an investigation into an experiment using hydrogen ...

    In my prediction I put the optimum temperatures as being about 40�C, but in my results table, and shown on my graph it was in fact about 25-30�C. This surprised me because enzymes are used to working in the body inside the stomach, which is about 30�C.

  2. Free essay

    effect of hydrogen peroxide on potatoes

    This gave us a rough estimate of what the results would be, and see if our predictions were right. It also told us how to conduct he experiment properly, and we worked out how we were going to measure the froth, which was to measure just the froth, and not the remaining liquid.

  1. Investagating the Action of the Enzyme Catalase On the Surface Area of a Potato.

    The steeper the line is the faster the reaction is. Where the line is horizontal, the reaction has stopped. At that horizontal point, the enzyme cannot decompose the substrate anymore. I would expect this type of graph for my results because at fist the rate at which the oxygen is

  2. Experiment to find out how temperature affects enzyme reactions

    After I have got my results I can then look back and see if I can prove my prediction. Method Apparatus: * Thermometer * Glass rod * Test-tube rack * Spotting tile * Stop watch * Beaker * Tripod * Measuring cylinder * Tripod * Gauze * Bunsen burner *

  1. Enzymes experiment. Do enzymes work faster or slower when the temperature is high?

    Then I would use one beaker to keep the hot or cool water inside it with the thermometer. It can also hold a lot of water in case I need to add some more water so I adjust the temperature.

  2. What influence does pH have on the enzyme Catalase?

    Holding at the same angle would stop the human error of moving it slightly and changing the results. Moreover, the measurements also required the use of rubber tube folded over as a seal to prevent any oxygen escaping. However, there was still a great chance of oxygen leaking out as

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work