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To find out how enzymes are affected by a variation in Temperature, the enzyme for the study is Catalase commonly found in potatoes. I will also find the optimum temperature of when the enzyme reacts quickest.

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Introduction

Aim: To find out how enzymes are affected by a variation in Temperature, the enzyme for the study is Catalase commonly found in potatoes. I will also find the optimum temperature of when the enzyme reacts quickest. Hypothesis: The hypothesis for this investigation is that when the temperature is increased, the amount of bubbles will increase in one minute so therefore the rate of reaction will increase. I believe this hypothesis to be true because of the collision theory that particles with more energy will move faster increasing the chance that a reaction will take place. Also the particles will have more energy so there will be more chance of the activation energy being reached. I also predicted that there will be an optimum temperature of around 40oC I predict this because body temperature is at about 37 oC. There will be an optimum temperature because enzymes denature at certain temperatures; denaturing is when the active site changes shape and the substrate cannot fit so it can be broken down. This is known as the lock and key theory. This causes that there is no reaction and no or very little bubbles will be counted. ...read more.

Middle

Concentration: This will be kept constant by always using the same concentration of liquids provided. pH: The pH will be kept constant by not changing the pH of the Hydrogen Peroxide and the Enzyme, Catalase. Volume of hydrogen Peroxide: This will be kept constant at 25ml by using a measuring cylinder. Apparatus: This will be kept constant by using my preliminary work r chose appropriate apparatus that will give me accurate results and using the same apparatus. Starting of Stopwatch: I have chosen to regulate the starting point of the stopwatch this will always be started at when the rubber bung with the delivery tube in it has been inserted. Apparatus and Chemicals used in experiment: 1x 25ml Measuring Cylinder Hydrogen Peroxide Water Potato 8x Test Tubes 1x Delivery Tube with bung Test Tube Rack 4x Water Baths (one at each of the following temperature 300C, 400C, 500C, 600C.) 2x thermometer 2x Cork Borers (one 8mm and the other of smaller dimension) 1x Stopwatch 1x Cutting Tile 1x Scalpel 1x Ruler Picture of Apparatus: Method: 1. I collected the apparatus, which is listed above I then, set it up as pictured above. ...read more.

Conclusion

I collected no anomalies in this experiment but this was due to the use of an accurate method and the processing of my results into rates of reactions and averages. This investigation could however be improved by taking a larger range of temperature at more frequent intervals this would give you also a more accurate optimum temperature and greater understanding of the process of Enzyme Action. I could not do this due to lack of time and resources. I may have collected data that could be inaccurate because when the potato and the Hydrogen peroxide are mixed, I had to push the bung and delivery tube into the top of the test tube and I had to start the stopwatch add and also count bubbles. I could have missed a bubble and also I could have started the stopwatch too late or too early and when the bung is pushed in this causes the air inside the test tube to be compressed. Therefore bubbles of air not oxygen can be produced and can be mistakenly counted as oxygen produced by the reaction. This could have been improved by having an assistant or using a n airlock system. By using these improvements in my investigation I could improve the accuracy of the results. Andrew Wright 5th Form Biology Coursework (Catalase) 1 ...read more.

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