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To find out if different concentration of hydrogen peroxide release different amounts of oxygen when in contact with catalase enzyme.

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Introduction

Catalase activity experiment Aim: to find out if different concentration of hydrogen peroxide release different amounts of oxygen when in contact with catalase enzyme. Risk assessment: Hydrogen peroxide is corrosive and can therefore irritate the skin and damage the eye if it comes into contact with these. To prevent this extra caution must be taken to avoid contact with the skin and goggles should be worn while carrying out the experiments. Other sharp implements such as the scalpel used for cutting the potato can be dangerous if the sharp side comes into contact with the skin as it could cut it. Prediction: I predict that as the concentration of the hydrogen peroxide decreases, so will the level of oxygen produced. I think this will happen due to collision theory. The collision theory is based on the principle that for a reaction to occur the reacting molecules must collide with each other with enough energy to react. The higher the temperature the more energy the molecules will have and the faster they will move, increasing the number of collisions and the chance that the collisions will lead to a reaction. In terms of enzymes, we are trying to get the substrate to collide with the enzyme and for the enzyme-catalysed reaction to take place. ...read more.

Middle

Boiling tube- container to put the slice of potato and the hydrogen peroxide in so as not to lose any of the oxygen. Thermometer- this is used to measure the temperature of the water ensuring the experiment is fair and the catalase functions at the same rate. Scalpel- this is used for cutting the potato. Test tube rack- used for holding test tube during experimentation. Stop watch- used for timing the amount of time that the catalase has been reacting with the hydrogen peroxide. White tile- this is used for cutting the potato on. Method: 1. Bring all the apparatus into the working area. 2. Cut the potato to an exact length of four centimeters long. 3. Measure the correct amount of hydrogen peroxide and water. Pour them into a boiling tube and shake to form a mixture. 4. Fill the measuring cylinder up with and also fill the plastic basin. Measure the temperature with the thermometer. 5. Put the potato into the boiler tube containing the mixture of water and hydrogen peroxide. Then put the cork with the tube attached to it on top of the boiling tube and insert underneath the measuring cylinder as quickly as possible, this will make sure that the minimum amount of oxygen will escape and provide the most accurate test results. ...read more.

Conclusion

0.5 1.0 1.1 1.3 1.3 1.5 1.8 1.9 2.0 2.0 0.3 0.3 0.8 1.0 1.0 1.3 1.5 1.5 1.8 1.8 0.0. 0.1 0.3 0.3 0.5 0.5 0.6 0.8 0.8 0.9 Average 0.3 0.5 0.5 0.9 0.9 1.1 1.2 1.3 1.5 1.5 Conclusion: The results show that as the concentration of hydrogen peroxide decreases, the amount of oxygen being released into the measuring cylinder also decreased. This is because the reaction was working at a slower rate due to the fact that the water had diluted the solution. The result of dilution means that there would be less enzyme to react with and therefore less oxygen would have been produced. The averages gave a good indication of the expected results. Evaluation: The results sometimes appeared slightly skewed which is shown on the graph as the anomalous results. When the cork on the boiler tube was removed some oxygen was lost, although the amount of oxygen lost will not have been a great amount meaning that the final result will not be too affected by it. However, this does mean that the method was not totally reliable. The water was not the exact right temperature for the enzyme to work at its maximum activity rate. These factors will have caused only minor changes to the results and so are therefore not too significant, this shows my overall method was relatively successful. ...read more.

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