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To investigate different concentrations of sucrose, between OM and 1M and to find out how potato chips in the solutions change in mass because of the process of osmosis.

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Introduction

An Investigation showing how the mass of potato chips changes due to osmosis PLANNING: Aim: To investigate different concentrations of sucrose, between OM and 1M and to find out how potato chips in the solutions change in mass because of the process of osmosis. Prediction: I think that the percentage change in mass of the potato will decrease as the concentration of sucrose in the solution is increased. I think this because "osmosis is the net movement of water through a selectively permeable membrane across a concentration gradient (from a high concentration of water to a low concentration of water)"- www.biology-online.org. This means that the holes in the membrane will allow water to pass in and out of the solution and the potato, depending on the concentration gradient of the two substances. In this case, when the water concentration is lower in the tissue, then the water moves into the tissue of the potato. This causes the potato to increase in mass. If there is a lower concentration of water in the potato, then the water moves into the tissue of the potato. This causes the potato to increase in mass. If there is a higher concentration of water in the potato, then the water moves out of the potato. ...read more.

Middle

* Cover the test tubes with a bungs and leave in a cool place for 50 minutes * Take the potato chips out from the sucrose and blot them in kitchen paper to absorb any sucrose. * Weigh them again and take an average reading Molarity of sucrose solution (M) Water (cm3) Sucrose solution (cm3) 0.0 10 0 0.1 9 1 0.2 8 2 0.3 7 3 0.4 6 4 0.5 5 5 1.0 0 10 OBTAINING EVIDENCE: Method: * I took a potato and cut out 21 cylinders from it, using a cork borer. * After laying all 21 pieces of potato on a tile, I used a scalpel to cut them down to 3 cm in length. * I put a blue pin in all the potato chips in the set of potatoes laid out for my second repeat and an orange pin in the chips for the third repeat. * I measured each potato chip separately on an electronic balance and recorded the results in a table. * I took 7 boiling tubes, put them in a test tube rack and placed amounts of sucrose solution and tap water as shown in the table above. ...read more.

Conclusion

The cell membrane in cells is semi-permeable and the vacuole contains a sugar/salt solution. So when a cell is placed in distilled water (high water concentration), the water will move across the semi-permeable membrane into the cell (lower water concentration) by osmosis, making the cell swell. This cell is now referred to as turgid. If done with potato cells the cells would increase in length volume and mass because of the extra water. If these potato cells were placed in a solution with a low water concentration, then the opposite would happen. Water would move out of the cell into the solution. In extreme cases the cell membrane breaks away from the cell wall and the cell is referred to as plasmolysed. The potato cells will have decreased in length, volume and mass. The greater the concentration of water in the external solution, the greater the amount of water that enters the cell by osmosis. The smaller the concentration of water in the external solution the greater the amount of water that leaves the cell. However, there will be a point where the concentrations of water inside and outside the potato cells are equal and this is when equilibrium is formed. At this point there will be no change in the length, volume and mass of the potato, as the net movement of water will be zero, no osmosis has occurred. Evaluation: ...read more.

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