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To investigate how surface area affects the method rate of the catalase reaction.

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Introduction

Enzymes Aim- to investigate how surface area affects the method rate of the catalase reaction. Equipment * Avocado * Borer * Knife * White tile * Goggles * Apron * Hydrogen peroxide (H2O2) * Conical flask * Trough * Water (H2O) * Ruler * Measuring cylinder * Delivery tube * Test tubes * Test tube holder * Bung * Thermometer * Stop watch Labelled diagram of the apparatus Method First we will set up the equipment as shown, and then we will put on some goggles and an apron to protect us from any spilt chemicals. We will then draw a results table; in the table we must state the surface area of each piece of avocado. We will carefully peel and the avocado using a knife, we will then use the corer to core five 50mm cylinders of avocado using a ruler. If the cylinders have dents or chips in them then we will have to state these for my results, in case there are any anomalous results. We will leave the first avocado cylinder as a 50mm core. With the second we will cut it into two pieces of equal size, with the third we will cut it into three fairly equal pieces, with the forth cylinder we will cut it into four fairly equal pieces, then with the last piece we will cut it into five equal pieces with the length of 10mm each. ...read more.

Middle

As the surface area increases, the speed of the reaction will increase therefore the gas will be produced faster, this is because most chemical reactions happen faster when there is more of the reactant to react with. Hypothesis Graph Using the apparatus safely and equipment properly While working all safety procedures were followed very carefully like the use of safety goggles and the careful use of knifes. I feel the measurements we took were fairly accurate as the results were very close to what we were expecting. The stopwatch was accurate to the nearest millisecond. We were not able to measure this accurately however so our time was accurate to the nearest second. Results table Trial 1 Trial 2 Trial 3 Avocado surface area mm� Volume of oxygen (O2) expelled in ml Average Volume Piece 1- 1178 11 (Different avocado to other 2 trials) 9 9 9.66? Piece 2- 1570.6 13 (Different avocado to other 2 trials) 15 10 12.66? Piece 3- 1963.2 14 (Different avocado to other 2 trials) 18 15(Dent in avocado) 15.66? Piece 4- 2355.8 17 (Different avocado to other 2 trials) 18 14(Dent in avocado) 16.33? Piece 5- 2748.4 19 (Different avocado to other 2 trials) 20 22 20.33? Analysing From my graph I have found generally, that as the surface area increases the volume of hydrogen peroxide increases too. ...read more.

Conclusion

we could have recorded the volume of oxygen every 180 seconds, this would therefore improve the accuracy of our graph which would lead to us having a more distinct and obvious pattern. The method could have been improved by using the same avocado for all three of the trials this would have made sure all the readings were accurate and precise. By doing this we could make the experiment more of a fair test. I feel the evidence is of a sufficient accuracy to support the conclusion, but I do not feel we had enough roar data to find out what happens to the volume of oxygen after the reaction has slowed down this could be found out however by collecting more data. I predict that if we did collect more data the results would then drop and decrease as the reaction slowed down to a stop. The results we recorded were what we expected apart from the anomalous result because the volume of oxygen only increased by 0.67ml. This could be solved though, by repeating that experiment again to give a more accurate result. The prediction however is still quite accurately followed by the results. If we were to do the investigation again we would probably look at altering another variable, for example the temperature of the hydrogen peroxide. This would give us extra patterns, similarities and gradient data to look for in the results. 1 Jo Elston 10Ben Science Coursework-Enzymes ...read more.

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