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To investigate how temperature affects the rate of the reaction with catalyse and hydrogen peroxide

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Introduction

Biology coursework Aim To investigate how temperature affects the rate of the reaction with catalyse and hydrogen peroxide Prediction I predict that at the lower and higher temperatures the reaction will slow to the point that the reaction does not occur. This is because the enzyme will de-nature and will not break down the hydrogen peroxide. I believe that the faster reactions will be at around 20�C because this is about the temperature that the potato will be experiencing in the soil and so its natural conditions will be in effect. Plan Apparatus * 2x 5ml� syringes * Potato puree * Hydrogen peroxide 10V * 100cm3� measuring cylinder * bee-hive shelf * bowl * ice cream tub * kettle * ice * conical flask * tube * water Method 1. ...read more.

Middle

a wide range of results from the temperatures that will be too cold for the catalyse and too hot so I can get the complete range. Using preliminary results I am using 10V hydrogen peroxide and 10cm3� of potato puree to get results that will not exceed 100cm3� and will be large enough to get more accurate and easy to measure results. Fair Test To make sure that the experiment is a fair test I will keep the same: * Volume of Potato puree * Volume of hydrogen peroxide * Time reaction is allowed to run on for * The same starting volume of oxygen in the measuring cylinder * Same length of tube I will also keep all the syringes in the water bath for 5mins to give the catalyse time to de-nature if it will do so. ...read more.

Conclusion

Evaluation In general the results I obtained were very reliable and kept with a distinct pattern. The only result that was an anomaly was at 60�C on the second test which gave 10 cm3; it crosses in to the 50�C results. To improve on my experiment I could have left the syringes in the water bath for longer to provide far more reliable results and make sure that the enzyme was de natured. I could also have done a test at just over 0�C to discover at what temperature the Catalyse denatures. To extend my results I could try different temperatures between 50�C and 60�C to find the exact temperature it denatures from at the higher temperatures. I could also see how the pH effects the reaction or how the concentration of hydrogen peroxide effect the rate of reaction. ...read more.

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