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To investigate how varying the temperature of the reaction between amylase and starch affects the rate of reaction.

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Introduction

An experiment to investigate the effect of temperature In the reaction between amylase and starch Aim To investigate how varying the temperature of the reaction between amylase and starch affects the rate of reaction. Prediction I predict that as the temperature of the reaction increases, so will the rate of chemical reaction. Scientific knowledge: As the temperature increases, the particles will have more energy to move around faster, resulting in more collisions which will lead to chemical reactions. This is linked to the collision theory, and as the temperature increases in this experiment, more collisions will occur between the amylase and starch, which should result in more starch being broken down into glucose. Method I will be heating up the starch and amylase separately to the first chosen temperature in a water bath so that the test tube is not directly heated. ...read more.

Middle

Predictions: What will happen at the different temperatures? In this experiment, I will be using the temperatures 0�C, 10�C, 20�C, 30�C, 40�C and 50�C. I predict that the rate of enzyme activity will be slowest at 0�C, because the substrates have less energy and will not collide with the enzyme as often and the collisions will not be as powerful. Therefore less starch will be broken down. As the temperature increases to 10�C and 20�C I think that the reaction will be faster as there is more energy. The rate of reaction will sharply increase up to about 37�C - 40�C, at which point the amount of activity will be the highest, and the iodine will turn to a light-brown colour in the shortest time. After this point, I think the rate will start to decrease because the enzyme is being altered by the heat and starting to be denatured. ...read more.

Conclusion

The amylase was breaking down more starch as the temperature increased because there was more energy present. Evaluation Overall, I feel that I gained the results I was hoping for at the start. There was a pattern that could be seen in the results, and the graph showed that there were no anomalous results. There was always the chance, though, of making a mistake in this experiment. The interpretation was left to human error as I had to judge when I felt the experiment had finished. I also had to try and make this judgement constant for each temperature, which is almost impossible. That is why, if I was to do the experiment again, I would use a log-it to record the amount of light getting through the iodine after the mixture had been added to it, which would then enable me to reach the end of the experiment at the same point each time. This eliminates the room for human error. ...read more.

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