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To investigate one factor which affects the way in which the enzyme catalase works.

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To investigate one factor which affects the way in which the enzyme catalase works. Aim: To investigate one factor which affects the way in which the enzyme catalase works. catalase Hydrogen peroxide ? oxygen + water 2H2O2 (aq) O2 (g) 2H2O(l) Background: There are three main ways to affect the way and speed that the enzyme catalase works. One is to change the temperature at which it is used. This affects the rate of reaction at which hydrogen peroxide is broken down because enzymes act like inorganic catalysts, and the rate of reaction doubles every 10?c within the temperatures of 0 and 40?, but above this temperature the enzymes effectiveness slows, and then stops as it becomes denatured. The optimum temperature is around 37?c, as this is the average body temperature and enzymes are designed to work in the body. For a reaction to occur, particles need to collide successfully. For a successful collision, molecules need to collide with enough energy to break the bonds. This is called the activation energy. The activation energy level, which is needed, can be lowered by use of a catalyst (in this investigation using the biological catalyst, the enzyme catalase) ...read more.


Fair test: To make this experiment a fair test, I will keep the mass of liver the same each time, and time for 30 seconds each time. I will also try to keep the temperature of the hydrogen peroxide solution at room temperature, as temperature can affect the rate of reaction. I will do this by measuring the temperature before the start of each reaction, not after as the reaction could be endothermic or exothermic. I will try and put the bung on as quickly as possible after the liver goes in so that none, or not a lot of the oxygen escapes without being measured. I will also not shake or stir the hydrogen peroxide and liver at all, as this could increase the rate of reaction due to the substrate particles coming in contact and so being broken down more by the liver. Preliminary work: Before I carried out this experiment I did some preliminary work to decide on what volumes, concentrations and masses to use. For example when using a lot of liver a vigorous reaction occurred so I decided to use 0.2g rather than any higher masses. I decided on the concentration of the hydrogen peroxide as well, as there is enough difference between each concentration for a change in the rate of reaction to be noticed. ...read more.


These can be seen best on my second graph which shows the rate of reaction against the concentration of the hydrogen peroxide. They are at 20% and 60%. These odd results are most likely due to human error. These could be improved by repeating the experiment more times to get more results to compare them with, or as I mentioned earlier by using more accurate equipment like the burette. Although there were these anomalous results, they still followed the general pattern, so I think that from my results I can draw a firm conclusion. To do further work on investigating factors which affect the rate of reaction of the breakdown of hydrogen peroxide, the experiment could be done, but instead of making concentration the variable, changing the temperature each time, or by changing the pH of the solution each time. (Both mentioned in greater detail in my background knowledge). Another thing that could make a more accurate experiment to make a firmer conclusion would be to use a wider range of concentrations hydrogen peroxide. As I mentioned earlier, the molarity of the 100% solution of hydrogen peroxide was 1.76 moles, so by using a higher concentration of hydrogen peroxide, it would have been easier to see the tail off of the rate of reaction at the end of the experiment. Clare Mann 11F 1 ...read more.

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