• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

To investigate one factor which affects the way in which the enzyme catalase works.

Extracts from this document...


To investigate one factor which affects the way in which the enzyme catalase works. Aim: To investigate one factor which affects the way in which the enzyme catalase works. catalase Hydrogen peroxide ? oxygen + water 2H2O2 (aq) O2 (g) 2H2O(l) Background: There are three main ways to affect the way and speed that the enzyme catalase works. One is to change the temperature at which it is used. This affects the rate of reaction at which hydrogen peroxide is broken down because enzymes act like inorganic catalysts, and the rate of reaction doubles every 10?c within the temperatures of 0 and 40?, but above this temperature the enzymes effectiveness slows, and then stops as it becomes denatured. The optimum temperature is around 37?c, as this is the average body temperature and enzymes are designed to work in the body. For a reaction to occur, particles need to collide successfully. For a successful collision, molecules need to collide with enough energy to break the bonds. This is called the activation energy. The activation energy level, which is needed, can be lowered by use of a catalyst (in this investigation using the biological catalyst, the enzyme catalase) ...read more.


Fair test: To make this experiment a fair test, I will keep the mass of liver the same each time, and time for 30 seconds each time. I will also try to keep the temperature of the hydrogen peroxide solution at room temperature, as temperature can affect the rate of reaction. I will do this by measuring the temperature before the start of each reaction, not after as the reaction could be endothermic or exothermic. I will try and put the bung on as quickly as possible after the liver goes in so that none, or not a lot of the oxygen escapes without being measured. I will also not shake or stir the hydrogen peroxide and liver at all, as this could increase the rate of reaction due to the substrate particles coming in contact and so being broken down more by the liver. Preliminary work: Before I carried out this experiment I did some preliminary work to decide on what volumes, concentrations and masses to use. For example when using a lot of liver a vigorous reaction occurred so I decided to use 0.2g rather than any higher masses. I decided on the concentration of the hydrogen peroxide as well, as there is enough difference between each concentration for a change in the rate of reaction to be noticed. ...read more.


These can be seen best on my second graph which shows the rate of reaction against the concentration of the hydrogen peroxide. They are at 20% and 60%. These odd results are most likely due to human error. These could be improved by repeating the experiment more times to get more results to compare them with, or as I mentioned earlier by using more accurate equipment like the burette. Although there were these anomalous results, they still followed the general pattern, so I think that from my results I can draw a firm conclusion. To do further work on investigating factors which affect the rate of reaction of the breakdown of hydrogen peroxide, the experiment could be done, but instead of making concentration the variable, changing the temperature each time, or by changing the pH of the solution each time. (Both mentioned in greater detail in my background knowledge). Another thing that could make a more accurate experiment to make a firmer conclusion would be to use a wider range of concentrations hydrogen peroxide. As I mentioned earlier, the molarity of the 100% solution of hydrogen peroxide was 1.76 moles, so by using a higher concentration of hydrogen peroxide, it would have been easier to see the tail off of the rate of reaction at the end of the experiment. Clare Mann 11F 1 ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Patterns of Behaviour section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Patterns of Behaviour essays

  1. The effect of aspirin on the action of bovine liver catalase

    Broken glass is disposed of into a special glass bin. It should not be put in a normal bin as anyone who handles the rubbish could get cut through the bin liner.

  2. The Effect of Catalase in the Breakdown of Hydrogen Peroxide

    Therefore; substrates bump into fast enzymes very rapidly, causing a faster reaction. Nevertheless; a temperature higher than body temperature can result in the denaturing of the enzyme itself. This is because the kinetic energy given by the heat caused the enzyme's active site to malfunction and thus; change shape.

  1. How Temperature Affects Catalase Activity.

    Now make the boiling tubes to stand in a section of the rack. 3. Now that the boiling tubes have been labelled with the appropriate information, fill 7 of the tubes with 15cm3 each of catalase solution and the other 7 with 15cm3 of hydrogen peroxide.

  2. Enzymes - show how substrate concentration affects the rate of reaction for an enzyme ...

    Another error in the preliminary experiment was that the temperature. This was because the temperature wasn't kept constant because if the initial temperature of water was 40�C then by the end of the experiment, the temperature will decrease gradually. This is because I did not use specific equipment which would


    Demonstrated below (table 8) are the figures gained from the average pH decrease for each concentration. Between the factor of 0% and 1% there are no differences shown. The pH average fall then decreases in 2% concentration. This contradicts earlier prediction and also opposes my other results, which show that

  2. An investigation to see the difference in the rate of reaction when catalyse is ...

    I can't actually control the temperature in the room with the equipment I am using because it isn't very advanced. But this won't effect the fairness of my investigation because I will do all my investigation on the same day with the same temperatures.

  1. Investigation into the initial rate of hydrogen peroxide decomposition when the enzyme catalase is ...

    Every 10 seconds for 2 minutes the O2 collected will be recorded so that the rate of reaction can be worked out. To alter the substrate concentration, the H2O2 will be mixed with water.

  2. To investigate and compare the effect of temperature on the activity of catalase in ...

    Substrate molecules that cannot find a vacant active site have to wait until the enzyme/substrate complex has released the products so the active site is once again free to take more substrate. BELOW: EFFECT OF SUBSTRATE CONCENTRATION ON THE RATE OF AN ENZYME-CONTROLLED REACTION.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work