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To investigate the effect of changing concentration of Hydrogen Peroxide (H2O2) on the enzyme catalase.

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Introduction

To investigate the effect of changing concentration of Hydrogen Peroxide (H2O2) on the enzyme catalase. Equation: - 2H2O2 catalase 2H2O + O2 Background information: - My background information on my experiment is that the substrate (H2O2) is by product of the chemical RX inside the body that is linked to the metabolism; the substrate is a toxic (poisonous) by product of the RX chemical, which kills and stops RX's. The H2O2 that we are going to use has been broken down into safer less toxic molecules and compounds. Enzymes are chemicals/catalysts. Catalysts alter the rate of reaction by making the rate of reaction faster. Catalase is a protein, which means this is an enzyme because all enzymes are proteins. Enzymes being proteins the work best at 37 degrees (body temperature) to much excess heat and it will denature the enzyme, the more enzymes you have the faster the rate of reaction will be. Lock and Key Theory + Catalase Substrate (Enzyme) (H2O2) Complex Enzyme Product Rate Max rate Rate Substrate concentration Enzyme concentration Substrate concentration Enzyme concentration reaches a Plateau plenty of Enzyme because all Enzyme Molecules to deal Molecules used up with substrate. so you add more Substrate is constant. H202 the rate stays the same. Plan - How are you going to do the experiment? In my experiment I am going to use the catalase yeast because it gives a faster rate reaction, also I will be using a solution of H2O2, which comes neat 6% and 40 volume. ...read more.

Middle

in a certain time (3min). This will tell me the rate of reaction (volume /time) What I think I will find out is that the higher concentration of the substrate will have the higher rate of reaction. This is because the higher concentration will have more activation energy, which will produce more collisions and harder collisions. This will make the particles gain more heat that will lead to the particles reaching the activation energy. This will happen quicker with the higher concentration. I will carry out the following method using 0.5 gram ball of yeast, 100cm3 of substrate and catalase in a conical flask with a bung attached via a tube to a large measuring cylinder full of water in a beaker filled with water. When the oxygen bubbles are produced they go through the tube in to the measuring cylinder where they are measured by the water they have substituted. O2 bubbles Measurement of O2 produced Stop clock Tube Conical flask Beaker Water Substrate (H2O2) Yeast Observation I will carry out 5 measurements I will carry out measurements from 1% to 6% I will take the following precautions to make sure my results are accurate such as always measure the correct amount of substrate and keep the conical flask at the same temperature all the time. Also I will make and stop the experiment dead on 3 minutes. I will wash the conical flask to rid any substrate and thoroughly dry any water that was in it so that my measurements were exact. ...read more.

Conclusion

I think these affected my results because: - The 0.5 gram of yeast could have been heavy which would make the rate of reaction faster or could have been a little less which would make the rate of reaction slower. Also the water in the measuring cylinder would have made the reaction go slower. The limitations in the equipment I used were: - The measuring cylinder had too many gaps in the measurements so we could not measure precisely and the stop clock had a delay so we got a slower time. I think these affected my results because: - We could not measure precisely how much hydrogen bubbles were produced and the results may have been slower in the delay of the stop clock. My anomalous results were in the 5% concentrated. I think these may have happened because some of the pupils diluted the substrate, which made the rate of reaction very slow. The changes I would make to my equipment and method if I did this investigation again are: - That I would have my yeast balls precisely measured and have a large measuring cylinder with small measurements for small precise measure. Also I would make sure the beaker and measuring cylinder were completely dry. What I would do to extend my investigation is: -to see if the pattern was the same if I used a gram ball of yeast instead of a 0.5 gram. Also I cold use bigger concentrations of hydrogen peroxide and in small concentrations like 1.5% then 2% and so on. Ross Murray ...read more.

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