• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

To investigate the effect of changing the total surface area of a potato containing catalase, on the enzyme activity when hydrogen peroxide is added.

Extracts from this document...

Introduction

Aim To investigate the effect of changing the total surface area of a potato containing catalase, on the enzyme activity when hydrogen peroxide is added. Background Information An enzyme is a biological catalyst, which is present in potatoes. Hydrogen peroxide is produced in all living cells. If it builds up, it is toxic and so it must be broken down in order to prevent this. Catalase is an enzyme found in all living cells. It speeds up the breakdown of hydrogen peroxide into two harmless substances, water and oxygen. The enzyme that I am using, catalase, is found in potatoes. It exists inside the cells and is released when the potato is cut open. Because it is from the potato, it is normally in a cold place when the potato is growing. Correcting pH or temperature imbalances will usually allow the enzyme to resume its original shape or conformation. Some substances when added to the system will irreversibly break bonds disrupting the primary structure so that the enzyme is inhibited permanently. ...read more.

Middle

6. After 5 minutes, record volume of oxygen, which has been produced. 7. Repeat experiment with the same sized piece of potato cut up into different numbers of pieces. Preliminary Experiments We conducted some preliminary experiments to distinguish the exact amount of hydrogen peroxide needed to ensure that the experiment was successful and that the reaction took part within the allotted 5-minute period. We found that this amount, after several attempt with other volumes, was 25cm�. Fairness To make it a fair investigation I will have only one variable, the number of pieces the potato is cut into. Room temperature will be kept the same although since we cannot control this, we will record any changes that may occur and take them into account when considering the conclusion. If the room temperature were to rise, it would affect the overall result. Before cutting the pieces up, we will insure that it begins sized 4cm by 1cm by 1cm. ...read more.

Conclusion

I had six results, which was just enough to support my conclusions, I would have done more to ensure that I had reliable results, but there was not enough time to do more tests, accurately. If I were to do the experiment again I would measure the potato pieces using a micrometer, which would provide far more accurately sized pieces and mean a much more accurate set of results. Also it would have been more accurate if I had someone to start the clock as it is difficult to mix the solutions and start the clock at the same time. To make the experiment more accurate I would have repeated the tests 3 times to make my results more reliable. My test was fair as I ensured that all the variables except the number of pieces of potato were kept constant for all the tests that I did. ?? ?? ?? ?? Heather R Davies 11MDM Biology Coursework 2000/2001 Investigating Enzymes C:\Documents and Settings\ckd\My Documents\essays\doc\after\1936.doc ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Life Processes & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Life Processes & Cells essays

  1. Marked by a teacher

    Effects of Surface Area on Catalase Activity in Potato.

    5 star(s)

    Controls Temperature - an increase of temperature will increase the rate of reaction until the optimum temperature of the enzyme is reached.

  2. The Effect of Surface Area on Catalase Activity in Potato

    As there is more substrate concentration, the more enzymes' active site will be used; increasing the substrate concentration wont necessarily increase the rate of reaction, as the amount of enzyme is the limiting factor. The graph below shows that the rate of reaction will be continuous up to a point where all the active sites of an enzyme are occupied.

  1. The Effect Of Changing The Surface Area Of Potato On The Breakdown Of Hydrogen ...

    Concentration of hydrogen peroxide The concentration of hydrogen peroxide has to be kept constant because a high concentration has an increased rate of reaction, and a low concentration has a lower one. Hence if different ranges were to have different volumes of this substrate, then different rates of reaction will

  2. Experiment to investigate the effect of Temperature on the enzyme activity of Pectinase

    Substrate concentration The graph is this time a curve dependent upon the substrate concentration. At a low substrate concentration there are a lot of unoccupied active sites causing the rate of reaction to be low. At a higher concentration of substrate enzyme-substrate complexes allowing more collisions to take place.

  1. The effect of hydrogen peroxide on catalase if you change the temperature.

    This means that the results of the experiment are presented in a clear and orderly fashion that allows patterns in the results to become more obvious. The rate of reaction was calculated by dividing 1000 by the time taken for the quantity of gas to be produced from the reaction.

  2. Investagating the Action of the Enzyme Catalase On the Surface Area of a Potato.

    I will also keep the same conical flask so that the width of the conical flask is the same for each experiment, which makes it fair for potatoes in the solution. This is because, if the bottom of the flask is wider, the potato will not be surrounded by the solution as much as when the flask is narrower.

  1. Investigate the effect of temperature on the activity of catalase (from potato)

    Arrange apparatus. 3. Get 250cm3 beaker and fill with water to about the 150cm3 mark with water at a specific temperature. This temperature can be reached by either cooling, via the use of ice, or by heating, via the use of a Bunsen burner.

  2. Experiment to investigate the effect of surface area on the rate of reaction.

    Wash off immediately. Diagram Brief Method and Results Table There were originally 2 methods to choose from that would present my results effectively: 1. Bubble Collection - Counting the number of O2 bubbles being produced. 2. Water Displacement - by O2 in the measuring cylinder.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work