Aneela Sirfraz 18/11/02
Investigation in to the effect
of enzyme concentration on
digestion of starch by amylase
Aim
To investigate the effect of enzyme concentration on digestion of starch by amylase
Hypothesis
The greater the concentration of amylase the bigger the circle produced due to greater digestion of starch.
Apparatus
2 petri dishes containing starch agar jelly
Bunsen burner
Mountain needles
Cork borers
Micropipette
7 test tubes
Test tube rack
0.5% amylase solution
Distilled water
Iodine solution
Felt tip pen
Ruler
Safety
Due to the use of the Bunsen burner I shall wear safety glasses and when not using it I shall put it on to the safety flame. Also whilst carrying out the experiment I shall be careful when using the mountain needles as they are very sharp.
Method
- Firstly on the back of the two petri dishes I shall divide each petri dish in to 4 parts and label each one with a concentration percentage.