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To investigate the effect of temperature on catalase.

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Introduction

How temperature effects the breakdown of hydrogen peroxide by the enzyme catalase Aim: To investigate the effect of temperature on catalase An enzyme is a protein molecule that speeds up chemical reactions in all living things, without enzymes these reactions would be to slow or would not happen. Enzymes are also useful because they cannot be used up and does not affect the reaction in any other way. My experiment will be testing the affect of temperature on catalase. I will need to try and keep these constant for the experiment to be fair: 1. PH: if the pH is too high or too low the enzymes will become denatured because of the loss of H+ ions at too high PH, and the gain of H+ ions when the pH is low. 2. Concentration of enzyme: The higher the concentration, the more successful collisions occur and the higher the rate of reaction. I will try to keep the same concentration of enzymes by using the same volume of potato. c) Surface area: If the surface area is larger there will be more successful collisions. I will try and keep this constant by measuring and cutting accurately. Kinetic theory states that when a substance is heated, energy is given to the particles and they vibrate faster. ...read more.

Middle

70 C, 80 C I will also be doing this experiment at room temperature but does not need a water bath Set up apparatus like diagram above Cut the potato so you will get 3 small pieces with the dimensions 5cm by 1cm by 1cm. Add 30cm of Hydrogen peroxide to each of the 3 test tubes by using the beaker. Put three thermometers in each test tube Put the three test tubes in the rack and put the rack in the 0 C water bath. Wait until the temperature reaches the temperature of the water bath Add one of the potato slices into one of the three test tubes and start your stopwatch After 2 minutes record the height of bubbles cm for that test tube and record the results. Rinse the solutions in the sink and put the potato in the bin Repeat this experiment two more times with the two other potato slices and take an average Repeat experiment with different potato slices in the other water baths at different temperatures and do the room temperature one by using the rack on the table. In order to observe the effect of temperature on catalase i will be maintaining the amount of oxygen released. The oxygen produces a froth, which I will measure in cm I calculated the average by adding the three results of the height of bubbles at that temperature and then dividing it by three. ...read more.

Conclusion

reached the desired temperature, the results were altered because the temperature was always raising hence the higher temperatures reached the optimum temperature of the enzymes. The results were not fair at all because of the above reason. I do not have enough evidence to draw a conclusion due to the fact that my results are not reliable or precise. The other reasons why my experiment did not go right were because the potato was not cut accurately, so the surface area or the amount catalase was not always the same. I thought that the pH would remain constant but it could of varied. If I were to do this experiment again I would: 1.Wait until the hydrogen peroxide reached the desired temperature and then put the potato in. This would make the test much more fairer as when the potato was in the hydrogen peroxide the temperature would remain the same. It would also be fairer because the temperature was supposed to be kept constant and instead was a variable. 2. I would turn the potato into paste and measure the volume. This way the amount of enzymes would be similar in each test. Or I would use a template to cut the potato, as this would give more accurate cutting. If I had just done number 1., the whole test would have been fairer and due to that, my evidence cannot support my conclusion. ...read more.

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