• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

To investigate the effect of temperature on enzyme activity. The enzyme used will be catalyse.

Extracts from this document...

Introduction

Experiment Plan Experiment: To investigate the effect of temperature on enzyme activity. The enzyme used will be catalyse. The enzyme catalyse: The enzyme catalyse is a biological enzyme. It is used to break down Hydrogen Peroxide, which is harmful. The reaction that occurs is: Hydrogen peroxide Water + Oxygen The activity of the enzyme catalyse can therefore be measured by the amount of oxygen (in the form of froth) that is produced. Prediction: The enzyme catalyse will have an optimum temperature of between 40�-45�. If the temperature rises above this temperature range or below this temperature range, the rate of enzyme activity will fall. This is because the enzyme catalyse is a protein enzyme and a biological catalyst, and the optimum temperature of most biological catalysts is between 40�C-45�C. If the temperature rises above this, the enzyme activity will slow down because the enzyme will be denatured. This means that the active site will be changed. Enzymes only work if the substrate fits into to the enzymes active site, like a lock and key (see diagram). The reaction then takes place and the product leaves. If the active site shape is changed the substrate will no longer be able to fit in (see diagram). This means that the reaction can no longer take place. As the temperature gets higher above 45�C, more enzymes will be denatured. The higher the temperature above 45�C, the slower the reaction rate. ...read more.

Middle

Temperature (�c) Volume of oxygen produced (cm ) 1 Volume of oxygen produced (cm ) 2 Volume of oxygen produced (cm ) Average Ice 10 9 3 6 Room 20 47 46 46.5 30 12 20 20 40 17 Not tested 17 50 5 4 4.5 60 3 Not tested 3 70 3 Not tested 3 The results that are highlighted grey are considered anomalies, so have been discounted from the average. Analysis Conclusion: The results show that the optimum temperature for catalyse to work at is 20�C. Looking at the initial part of the reaction (see graph), between 10 and 20�c the gradient of the graph was quite steep. Between 20�c and 30�c the gradient of the graph was also steep, but after 40�C the line begins to level off. These results partly do and partly do not support the prediction. As the prediction stated, the reaction time was slower at the coldest and hottest temperatures. This is because at lower temperatures the enzymes do not move with as much energy. This is because of kinetic theory, which states that the more heat energy a particle has, the faster, and with more energy, it will move. At 10�C the particles of the catalyse have less kinetic energy, so collide with the particles of the hydrogen peroxide with less energy. Therefore a reaction is less likely to take place, and less oxygen (in the form of froth) ...read more.

Conclusion

The results for this experiment gave the optimum temperature for the catalyse as 20�C. Although I did suggest a reason for this in my conclusion, it is possible that my prediction was correct and that that result was a n anomaly. In order to ensure a greater reliability of results, I should have repeated each experiment 3 times. Unfortunately, there was not enough time to do this. In addition, when I plotted my results in a rough graph, (see below) I noticed that the result for 30�C did not fit in with the trend of the data. I therefore looked at my data for the result for 30�C, and decided that the result 12 cm was an anomaly. It was possibly caused by too much potato being left in the syringe, meaning there was less catalyse in the experiment. I believe I collected a suitable range of results for this experiment, as the results I collected enabled me to see a pattern clearly. However, as I believe that the pattern shown by my results would continue beyond the range specified, If I repeated this investigation I would develop it by also using the temperatures 0�C, 80�C, 90�C and 100�C. Another good way of developing this experiment would be to have the temperatures used closer together, e.g. 20�C, 25�C, 30�C, etc. Having closer together temperatures would make it clearer to see at what temperature which denaturing took place. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Life Processes & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Here's what a teacher thought of this essay

4 star(s)

****
A good understanding of the theory behind the investigation is shown but some weaknesses in the method followed are evident.

Marked by teacher Adam Roberts 10/05/2013

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Life Processes & Cells essays

  1. Marked by a teacher

    Osmosis in Potato Chips

    5 star(s)

    This means that there is a higher pressure inside the cells than there is outside the cells and so the cells expand like balloons and become harder. I think that eventually there comes a point where no more water can enter the potato cells because turgor pressure is too high

  2. Marked by a teacher

    Isotonic Point of Potatoes

    5 star(s)

    These could have been random or systematic errors, and have been identified below: Possible sources of error: * The mass may not have been measured accurately as the air conditioner was on, which resulted in the continuous fluctuation of the reading on the balance.

  1. Marked by a teacher

    Plan: The effect of the end product, phosphate, on the enzyme phosphatase

    5 star(s)

    which produces phenolphthalein and phosphate when reacts with phosphotase in a buffer solution. The reaction was carried out in 5 test tubes with different concentrations of sodium phosphate added to the buffer in each beaker and incubated in the same water bath for 20 minutes.

  2. Marked by a teacher

    The Effect of Temperature on Amylase

    3 star(s)

    -Stopwatch -Test tube rack -Amylase solution -Starch -Beaker (5) -Ice -Hot water -Alcohol thermometer (accurate to 3.0?C) Diagram: Fig 1.0 Fig 2.0 Fig. 3.0 Method: 1. Using a syringe (accurate to� 0.1cm�) put 15cm� of starch into 5 test tubes. Put the test tubes in 5 different beakers filled with water of different temperatures (20?C, 40?C, 60?C , 80?C).

  1. A investigation into the effect of inhibitor concentration on the enzyme catalase.

    +3.48 330 +3.3 +3.96 360 +3.8 +4.56 390 +4.3 +5.16 420 +4.8 +5.76 450 +5.2 +6.24 480 +5.6 +6.72 510 +5.9 +7.08 540 +6.2 +7.44 570 +6.4 +7.68 600 +6.6 +7.92 Total Increase =+6.6 Total Increase =+7.92 0cm of Distilled Water 10cm of Lead Nitrate Solution TIME FROTH LEVEL VOLUME

  2. See the effects of amylase on starch at different temperatures and to find at ...

    and no rate of reaction at 60�C at which the enzymes had denatured and the active site was damaged, which means the enzymes could not react and break down the substrate (starch). It shows a pattern of decreasing rate of reaction, because the higher the temperature the more the rate of reaction decreased (only after 30�C)

  1. Factors affecting the activity of potato catalase on hydrogen peroxide.

    If the temperature increases the kinetic energy will speed the reaction. To get better results we could have increased the amount of hydrogen peroxide, making it a 110 % solution, which would then give us more reactions and better results.

  2. The aim of this experiment is to demonstrate that the substrate Hydrogen Peroxide will ...

    Total Surface Area (cm3) Volume of Oxygen Produced (cm3) Time (minutes) 1 x 3 + 2 x 1 24.402316 6.8 0.5 1 x 3 + 2 x 1 24.402316 6.4 1.0 1 x 3 + 2 x 1 24.402316 6.1 1.5 1 x 3 + 2 x 1 24.402316 6.0 2.0 1 x 3 + 2 x 1 24.402316

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work