To investigate the effect of temperature on the rate of anaerobic respiration in yeast.

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Biology Coursework: Planning

Aim: To investigate the effect of temperature on the rate of anaerobic respiration in yeast.

Prediction: I predict that the yeast will respire faster up to the temperature of 50°C. This is because for yeast to anaerobic respire it uses enzymes and enzymes are denatured past 50°C. Because of this I think that the yeast will respire fastest at 50°C and once past that temperature it will start to slow down and once all of the enzymes are denatured. I think that the fastest rate of respiration will be 50°C because every 10°C rise in temperature doubles the rate of respiration, but after the maximum temperature every 10ºC rise the rate of reaction will decrease by the same amount it increased.

Method: To do this experiment I will be using the following pieces of equipment, a boiling tube, a delivery tube, a water bath, a thermometer an solution of 0.1g of yeast to 20cm3 of water, sugar, cooking oil, a timer, a beaker, a measuring cylinder and hot water. I will set up the equipment as follows:

I will be given the yeast-water solution and will be using a top pan balance to measure