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To investigate the effect of varying sucrose (sugar) concentration on potato cells and to find out the concentration of sucrose in the potato cells.

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Introduction

Aim To investigate the effect of varying sucrose (sugar) concentration on potato cells and to find out the concentration of sucrose in the potato cells. Back round info Osmosis is defined as 'the movement of water molecules from an area of high water concentration to an area of low water concentration, across a semi-permeable membrane. In a high concentration of water the amount of solute (sugar) is low. This is called a dilute solution. In a low concentration of water the amount of solute (sugar) is high. This could be called a concentrated solution. When two such solutions are divided by a semi-permeable membrane. The water will move from the area of high concentration to the area of low concentration, until both sides are equal or have reached equilibrium. This can be seen in living cells. The cell membrane in cells is semi-permeable and the vacuole contains a sugar/salt solution. Factors and fair testing 1. Volume of solution 2. Surface area of potato used (Length and shape) 3. Temperature 4. Mass of potato 5. ...read more.

Middle

Scales Knife Test tube Test tube holder Pen Sticky label Method A range of sugar solutions will be prepared with concentrations from 0 M to 1.0, in intervals of 0.2. Sections of a potato will be cut using a knife to equal lengths (this will be 4cm in length) this will keep the surface area constant and their mass measured.10 ml of each concentration of sugar will be placed in three test tubes and labelled. We will then weigh the potato section on some scales. To each test tube a cut piece of potato will be added. These will be left for 24hours to allow the osmosis to take place. Then the potato pieces will be removed, the surface solution removed using towels and weighed. Three readings are taken for each concentration in order to get an average. The results will then be added to our result table. Prediction The greater the concentrations of water in the external solution the greater the amount of water that enters the cell by osmosis. ...read more.

Conclusion

You can see at concentration above 0.2 M to 0.4 M there were no further water loss/gained in the potato suggesting that the balance between the potato and the solution are equal. After this the potato did not gain any more water instead it started to lose water. Evaluation The graph obtained in the results has a strong resemblance to prediction I made. This suggests that the experiment was fairly accurate. The accuracy of the investigation was adequate, however the concentrations were measured using a measuring cylinder and so may not have been accurate (we also had to rush them). Also when the potato was dried to remove surface liquid it was not necessarily done the same on each potato, a more accurate way of drying would improve the accuracy further. Although 3 repetitions of each concentration are sufficient, there was a possible anomalous result (circled on graph). This part of the investigation would need to be repeated Other investigations could include using different varieties of potato or different plant tissues e.g. carrot. This would show us more of the higher to less concentration theory. ...read more.

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