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To investigate the effects of increasing yeast concentration on the rate of Hydrogen Peroxide decomposition

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Introduction

To investigate the effects of increasing yeast concentration on the rate of Hydrogen Peroxide decomposition Diagram Method 1. Weigh 0.1g of dry yeast powder into a conical flask using a top pan balance 2. Pour 40cm3 of Hydrogen Peroxide 10vols in to a measuring cylinder 3. Submerge a 100 cm3 measuring cylinder into a water bath ensuring no water escapes and no air bubbles form inside. 4. Add the Hydrogen Peroxide to the yeast and place the bung in the conical flask at the same time start the stop watch (try to minimise gas loss). Safety Wear goggles and wash your hands they come nt contact with Hydrogen Peroxide as it is highly irritant. ...read more.

Middle

When joining molecules, enzymes bring together the molecules to make it easier for them to be joined. As shown by the graph the rate of reaction increases as the mass of yeast is increased. This shows that an increase in concentration of an enzyme increases the rate of reaction. This is because there are more enzymes available for the substrate to collide so it is more likely a successful collision will take place and an enzyme-substrate complex will form thus increasing the rate of reaction. Evaluation I feel my results are fairly accurate however I think that the relationship between the rate f reaction and the enzyme concentration is more linear then the graph and the trend line suggest; this is something I would like to investigate I could also make results more reliable if I were to repeat the experiment several times (at least two more times). ...read more.

Conclusion

If I were to use a larger volume syringe than the 100 cm3 measuring cylinder to collect the gas I would be able to see more accurately when final volume gas was produced because sometimes this was hard to do when the reaction was going so fast. I could extend the experiment by testing higher masses of yeast in order to see if this relationship between rate of reaction and enzyme concentration increased forever or whether it is ever limited by the substrate concentration. However to do this I would have to decrease the volume of hydrogen peroxide used because this created far to much gas thus I would need to scale it down. ?? ?? ?? ?? Mark Martin Biology Experiment 07/05/2007 ...read more.

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