• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

To investigate the effects of increasing yeast concentration on the rate of Hydrogen Peroxide decomposition

Extracts from this document...

Introduction

To investigate the effects of increasing yeast concentration on the rate of Hydrogen Peroxide decomposition Diagram Method 1. Weigh 0.1g of dry yeast powder into a conical flask using a top pan balance 2. Pour 40cm3 of Hydrogen Peroxide 10vols in to a measuring cylinder 3. Submerge a 100 cm3 measuring cylinder into a water bath ensuring no water escapes and no air bubbles form inside. 4. Add the Hydrogen Peroxide to the yeast and place the bung in the conical flask at the same time start the stop watch (try to minimise gas loss). Safety Wear goggles and wash your hands they come nt contact with Hydrogen Peroxide as it is highly irritant. ...read more.

Middle

When joining molecules, enzymes bring together the molecules to make it easier for them to be joined. As shown by the graph the rate of reaction increases as the mass of yeast is increased. This shows that an increase in concentration of an enzyme increases the rate of reaction. This is because there are more enzymes available for the substrate to collide so it is more likely a successful collision will take place and an enzyme-substrate complex will form thus increasing the rate of reaction. Evaluation I feel my results are fairly accurate however I think that the relationship between the rate f reaction and the enzyme concentration is more linear then the graph and the trend line suggest; this is something I would like to investigate I could also make results more reliable if I were to repeat the experiment several times (at least two more times). ...read more.

Conclusion

If I were to use a larger volume syringe than the 100 cm3 measuring cylinder to collect the gas I would be able to see more accurately when final volume gas was produced because sometimes this was hard to do when the reaction was going so fast. I could extend the experiment by testing higher masses of yeast in order to see if this relationship between rate of reaction and enzyme concentration increased forever or whether it is ever limited by the substrate concentration. However to do this I would have to decrease the volume of hydrogen peroxide used because this created far to much gas thus I would need to scale it down. ?? ?? ?? ?? Mark Martin Biology Experiment 07/05/2007 ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Patterns of Behaviour section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Patterns of Behaviour essays

  1. Factors Affecting the Rate of Catalytic Decomposition of Hydrogen Peroxide.

    Time taken (s) 22 9.1 Inference: concentration increases the volume of gas collected, there is still too much catalyst being used and not enough volume of H202 (you can tell this because the rate of reaction is too low - it reaches terminal mass too early)

  2. Effect Of Substrate Concentration On The Activity Of Catalase

    = 2.62 12.65/300 = 2.53 (2.62+2.53)/2 = 2.58 40 13.4/300 = 2.68 13.5/300 = 2.7 (2.68+2.70)/2 = 2.69 50 5.50/300 = 1.1 4.85/300 = 0.97 (1.10+0.97)/2 = 1.04 60 2.85/300 = 0.57 2.35/300 = 0.47 (0.47+0.57)/2 = 0.52 70 0.70/300 = 0.14 0.6/300 = 0.12 (0.14+0.12)/2 = 0.13 Arefin Khan

  1. The Effect of Catalase in the Breakdown of Hydrogen Peroxide

    Insert a delivery tube up the burette; this must be connected to a stopper. 8. Using a pipette, extract the liquid from the bottom of the tube and place into a measuring cylinder to determine a percentage of the catalase that you want to test (measurements must be up to 10cm3).

  2. Investigation into the initial rate of hydrogen peroxide decomposition when the enzyme catalase is ...

    Enzyme Concentration Independent - Enzyme concentration causes there to be a fast initial rate of reaction as there are lots of free active sites for the substrate to collide with. It is unlikely, with a higher concentration of enzyme than substrate, that the substrate will be queuing, and this means that the reaction will happen faster.

  1. The effect of concentration on the activity of catalase.

    There are no ethical emplications to the treatment of living organisims as this was not a field experiment and was carried out in the laboratotry. If any glass were to break or any chemicals spilt, it shall be cleared away immediately.

  2. To investigate the effect of varying the masses of white sugar and yeast and ...

    It was fluid and yellow. Nowadays, yeast is made commercially on a large scale. The yeast used in this study was bought at a supermarket with the yellow lumps done up in paper and compressed for convenient handling. Yeast is a plant, according to the biologists, and is capable of reproducing itself.

  1. Factors Affecting Enzyme Activity

    As a control for this experiment I would conduct one of the experiments at a neutral pH due to the fact that catalase is found in a neutral pH. Although this would be a relatively easy factor to investigate I have chosen not to as I have a very limited knowledge of pH buffers.

  2. Investigate the effect of changing substrate concentration on the rate of the reaction between ...

    The higher the enzyme concentration, the faster the rate of reaction because if extra enzymes are present, the substrate will be able to get catalysed faster. More enzymes per cm3 will lead to more collisions between the substrate molecules and enzyme molecules, every second. Thus, a faster rate of reaction.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work