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To investigate the heart rate of Daphnia when added to certain liquids.

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Introduction

Jack Downes To investigate the heart rate of Daphnia when added to certain liquids. I will add ethanol to decrease the daphnia's heart rate and use red bull to increase the daphnia's heart rate. I will have to use many different daphnia, obviously, because if I don't the daphnia I use will already be contaminated. I predict that too much, Red bull or too much alcohol will kill the daphnia's. A daphnia is a see through water flea; these small fleas are food for small fish. The daphnia is see through but is too small to be seen by the naked eye, but when under a microscope you can see everything that is working in the daphnia. This is why we will be using daphnia for these experiments, they are seeing through and we can count the heartbeats. ...read more.

Middle

I will keep the room temperature the same, every time I carry out this experiment. I will have to use a different daphnia every time I add more ethanol or red bull. I am going to use five different concentration ranges from 10% down to 0.001% of each drug watered down. I will get concentration levels by: 1cm cubed of ethanol+9cm cubed of H20=10% of ethanol 1cm cubed of first solution+9cm cubed of H2 0=1% of ethanol and so on find 0.1%, 0.01% and 0.001% % Concentration Red Bull Test one Test two Test three 0 246 264 258 0.001% 318 318 300 0.01% 378 348 354 0.1% 470 486 462 1% 696 672 678 10% Dead Dead Dead Carried out the experiment by counting heart beats over 10 seconds and then worked out heart rate by multiplying them by 6. ...read more.

Conclusion

If I was to do this experiment again I think I would try and get all the practical done in one lesson because when you are working through only the pone lesson you are more unlikely too make a big mistake as you have been testing the whole hour but with spreading it over a few lessons I was more likely to change little things and make mistakes. I feel that over all my experiment went pretty well, there were no real big mistakes in my results. My conclusion is pretty correct I do believe my results would have been more interesting had I done them all in the same lesson. To improve this experiment I would make the dosages of both red bull and ethanol because I have no results for 10%, due to it being to strong for the little daphnia. ...read more.

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