A daphnia
For this experiment I will need:
- Daphnia
- Cavity slide
- Small amount of water
- Microscope
- Lamp
- Stopwatch
- Alcohol (ethanol)
- Red Bull
- Test tube
- Holder for test tube
- Measuring cylinder
- Pipette
I will use the pipette for collecting the daphnia and placing them on the cavity slide, which will then be placed under the microscope.
I will carry out the experiment three times to make sure that my results are as accurate as they can be. I will keep the room temperature the same, every time I carry out this experiment. I will have to use a different daphnia every time I add more ethanol or red bull. I am going to use five different concentration ranges from 10% down to 0.001% of each drug watered down. I will get concentration levels by:
1cm cubed of ethanol+9cm cubed of H20=10% of ethanol
1cm cubed of first solution+9cm cubed of H2 0=1% of ethanol
and so on find 0.1%, 0.01% and 0.001%
Carried out the experiment by counting heart beats over 10 seconds and then worked out heart rate by multiplying them by 6.
My results were pretty much what I had expected I thought that too much of either of the chemicals would kill the daphnia.
My graphs show the averages of each concentration number for before they had drugs the axis has gone from 0% to 0.001% in the same spacing as 1% to 10% because otherwise the results would all be crammed up on the end of the graph.
It shows there is a progressive decrease for alcohol. This line on my graph is a curve instead of a straightline.
I conclude that my tests were fairly fair but when in my tests mistakes were made either too much of either one of the chemicals was used or not enough. If I was to do this experiment again I think I would try and get all the practical done in one lesson because when you are working through only the pone lesson you are more unlikely too make a big mistake as you have been testing the whole hour but with spreading it over a few lessons I was more likely to change little things and make mistakes.
I feel that over all my experiment went pretty well, there were no real big mistakes in my results. My conclusion is pretty correct I do believe my results would have been more interesting had I done them all in the same lesson.
To improve this experiment I would make the dosages of both red bull and ethanol because I have no results for 10%, due to it being to strong for the little daphnia.
