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To investigate the impact of varying the concentration of sucrose solution has on the mass of potato.

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Introduction

Plan ---------------------------------------------AIM------------------------------------------------- To investigate the impact of varying the concentration of sucrose solution has on the mass of potato. ---------------------------------------BACKGOUND---------------------------------------------- SUCROSE Sucrose is a non-reducing, disaccharide sugar used in sweetening, being obtained from the juice of the sugar cane and from sugar beet. Sucrose (C12 H22 O11) is formed by a condensation reaction between Fructose and Glucose. POTATOES Potatoes are natural forms of carbohydrate, which are rich in starch. OSMOSIS Osmosis is the movement of a solvent (usually water in biological systems) through a differentially permeable membrane from a solution that has a high water concentration to one with a low water concentration and high solute concentration. Diagram 1: osmosis. WATER POTENTIAL Water potential is the tendency of a cell to draw in water from outside by osmosis with the water moving from a higher to a lower potential. Since pure water at one atmosphere has a water potential of zero, cells drawing in water have a water potential of less than zero. Thus, zero cells drawing in water have a water potential of less than zero. Thus water potential is measured as a negative value. ------------------------------------------HYPOTHESIS ----------------------------------------- With the increase in the concentration of sucrose there will be a net movement of water from the potato to the surrounding solution providing the potato has a higher water concentration (potential) ...read more.

Middle

The conclusions of the preliminary investigation will be used to write a suitable method for the final experiment. The results of the preliminary experiment proved that concentrations above 1m, should not used because they do not give accurate enough results and there is not enough water within the potato to be diffused outwards when the sucrose solution is very high. ------------------------------------ACTUAL INVESTIGATION------------------------------------ APPARATUS * Ruler * Cork Borer * Knife * Cutting tile * Filter Paper * Balance * Test tubes x 5 * Measuring Cylinder * Sucrose Solutions: 0m (distilled water), 0.1m, 0.25m, 0.5m, 1m * Potato * METHOD The diameter of the cork borer was measured and recorded. The cork borer was then used to cut five cylindrical pieces of potato each five centimetres long. A cylinder of potato was taken and cut into five pieces of length 1cm. These were weighed, their mass was recorded and they were added to a labelled test tube containing 20ml of distilled water. The test tube was then bunged and the time was recorded. This process was repeated with the other potato cylinders but sucrose solutions of 0.1m, 0.25m, 0.5m and 1m were used in place of the distilled water. The potato pieces were left in the solutions for 24 hours, they were removed and the time was recorded. ...read more.

Conclusion

To expand this experiment other variables in the experiment could be changed for example instead of changing the weight of the potato the type of the potato could be changed. For example 'New potatoes', 'King Edwards' etc. could be compared. Temperature could also be changed for example the samples could be placed in different water baths and brought up to different temperatures to see if temperature played its part in the osmosis of potatoes. 5 sets of 5 potatoes could be placed in water baths at 10oC, 20oC, 30 oC, 50 oC and 60 oC. Then leave them for 24 hours making sure all the variables in the first experiment still apply however just using one molar solution. Then after 24 hours the samples would be re weighed and results recorded. I would expect that at high temperatures the potato samples would osmose the most. This is because at high temperatures the solutions water molecules would move faster and therefore equal the concentration faster. A preliminary experiment could be set up beforehand to find out how long the experiment should be kept going because if the concentration of the potatoes equalises then the weight of the potatoes will be almost exactly the same. Other solutions could be used as well, e.g. glucose solution. I was not disappointed with my investigation because it achieved accurate results ...read more.

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