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To see how the concentration of hydrogen peroxide affects the rate of reaction with catalase

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Introduction

Biology Coursework Aim To see how the concentration of hydrogen peroxide affects the rate of reaction with catalase Background Information Catalase is an enzyme found in all living cells in the body. They perform certain reactions inside the cells and act as a catalyst. When hydrogen peroxide builds up inside cells as oxygen diffuses into the cell, catalase is a catalyst to break down the hydrogen peroxide so water and oxygen is produced. My investigation is to find out if the molarities of the reactants affect the rate of the reaction. During these experiments we will use hydrogen peroxide and we shall make catalase beads as shown in the diagram. We made the beads before hand for our preliminary work and method for using to react with the hydrogen peroxide. These beads were made by mixing 7ml of catalase in sodium alginate. Sodium alginate is a gel like substance and is one of the forms of entrapment used for immobilisation. Then we encased this mixture by putting small drops into calcium chloride, which encased them in a layer of calcium. ...read more.

Middle

Prediction My prediction is based on my preliminary work and the collision theory that states that the higher concentrations should make it react faster. The molarity will have this effect because with a greater concentration of hydrogen peroxide, more molecules of hydrogen peroxide will be present to react with the catalase inside the bead. This is why it should react quicker. My preliminary results also agree with the collision theory and it is for these reasons that I predict that higher concentrations should make it react faster. Variables Independent variable My independent variable is the change in molarity of the hydrogen peroxide. Dependant variable My dependant variable will be the time it takes for the bead to return to the surface. Control Variable The bead must have roughly the same amount of chemicals in each one. Each bead must be roughly the same size. One bead cannot be used again because it would have already reacted and got oxygen in it from the very start. There will be three beads used for each molarity to get a good average. ...read more.

Conclusion

It is the smooth curve, which distinguishes this on the graph. Evaluation From my results I have found that the higher the concentration of Hydrogen Peroxide, the quicker the reaction rates, producing oxygen. I have succeeded in what I planned to do, which was to find out how the concentration of hydrogen peroxide affects the rate of reaction for the enzyme catalase. The results I got were what I had expected and predicted and I only got one anomalous result. The results I got showed a clear curve on my graph so I was fairly happy with them. My experiment worked well although if I were to do it again I could do certain things differently in order to gain more accurate results. . More accurate measurements could have been used as the measuring cylinders used were only to either every 0.5cm2 or 1cm2. This is not really very accurate. Using a gas syringe, which measures much more accurately, could have solved this. I would repeat the experiment more times as this would give me a more accurate average and I would also uses more different values in order to get a more detailed outlook on how the concentration of hydrogen peroxide affects the rate of catalase activity. This may prevent any more anomalies occurring again. essaybank ...read more.

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