• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Vary an independent factor to see whether it affects the activity of the enzyme Catalase, using Hydrogen Peroxide (H2O2) and a potato solution (which has Catalase in it).

Extracts from this document...

Introduction

Catalase Investigation By Christopher Stylianou Aim To vary an independent factor to see whether it affects the activity of the enzyme Catalase, using Hydrogen Peroxide (H2O2) and a potato solution (which has Catalase in it). Factors All the independent variables, which could affect the investigation of Catalase, are: * The temperature of the enzyme: By changing the temperature the enzyme is working at, it might affect the activity of the enzyme Catalase. * The potato surface area: By changing the surface area of the potato, this might affect the activity of the enzyme Catalase. * The mass of the potato: By changing the amount of potato used in the experiment, it might affect the activity of the enzyme Catalase. * The concentration of H2O2: By changing the concentration of H2O2, this might affect the activity of the enzyme Catalase. * The concentration of the potato solution: By changing the concentration of the solution by using water, it might affect the activity of the enzyme Catalase. * The temperature of H2O2: By changing the temperature of H2O2 using water baths etc... this might affect the activity of the enzyme Catalase. * The pH of the potato solution: By altering the pH of the potato solution, it could affect the activity of the enzyme Catalase. * The volume of H2O2: By changing the volume of H2O2, it might affect the activity of the enzyme Catalase. ...read more.

Middle

This is because the average optimum temperature of Catalase is around 40 - 45�C. Anything below that temperature will take longer to produce a certain amount of gas and any temperature above this will denature the enzyme, so it will have no affect on the result. I have worked this out using my research above and my general knowledge. I have carried out a preliminary experiment to test out a plan and get an idea where my range should be for the real investigation. I decide to time how long the temperatures 30, 40 and 50�C would take to produce 50ml of Oxygen, using a solution of 5g of potato with 15ml of water, and then reacted with 20ml of Hydrogen Peroxide. Here are my results: Temperature �C Time Taken (mins/sec/10th sec) 30 5:30.45 40 3:31.21 50 4:23.54 The results show that 40�C is the shortest time to produce 50ml of gas. Using these results I can draw a preliminary graph of what the results should look like. I have decided to use a range of 20, 25, 30, 35, 40, 45 and 50�C temperature for the investigation to get a good range of accurate results. I am going to see how long it takes to produce 50ml of Oxygen using 5g of Potato, 15ml of Water and 20ml of Hydrogen Peroxide. Plan: - Apparatus: - Potato Solution: 35g of Potato and 105ml of Water 20ml Hydrogen Peroxide Water baths at 20, 25, 30, 35, 40, 45 and 50�C Burette Burette Clamp Thermometer Stand Timer Conical Flask Delivery tube Food Processor Scalpels Scales (with tray) ...read more.

Conclusion

* Make sure the scales start on 0.00 grams * The temperature of the potato solution is correct and does not change during an experiment. * Make sure the pH of the potato solution does not change as well as it can affect the experiment. To stop this from happening make sure you use the same potato solution for each experiment. Safety: - To make it a fair test you must carry out these precautions: - * Wear Safety Goggles at all times. * Bags and Stalls are put on the sides of the room, out of the way of any experiment. * The normal Science Labs rules must be obeyed. * Be careful with harmful substances. * Watch out for Glass objects, which might get broken. Results: - * = Anomalous Result Experiment 1 Temperature of Catalase (Potato Solution) �C Time taken to produce 50ml of Oxygen (mins/sec/10th sec) 20 7:47.54 25 * 7:03.12 * 30 5:20.19 35 4:00.46 40 3:24.38 45 3:57.56 50 4:24.54 Experiment 2 Temperature of Catalase(Potato Solution) �C Time taken to produce 50ml of Oxygen (mins/sec/10th sec) 20 7:42.01 25 6:21.15 30 5:31.41 35 4:15.06 40 3:26.24 45 3:67.37 50 4:31.46 Experiment 3 (Repeat for Anomalous) Temperature of Catalase(Potato Solution) �C Time taken to produce 50ml of Oxygen (mins/sec/10th sec) 20 - 25 6:32.51 30 - 35 - 40 - 45 - 50 - Average Results Temperature of Catalase(Potato Solution) �C Time taken to produce 50ml of Oxygen (mins/sec/10th sec) 20 7:44.78 25 6:26.85 30 5:26.03 35 4:07.76 40 3:25.31 45 3:62.47 50 4:28.00 ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Life Processes & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Life Processes & Cells essays

  1. Influence of pH on the activity of potato Catalase

    room for error, as the groups were taking the reading of how long it took the manometer to move 3cm, we were able to compile the data in graphs and result tables. However not all the group managed to complete the experiment and therefore there a many spaces in the table of results.

  2. What influence does pH have on the enzyme Catalase?

    The best model of enzyme function is imagining the enzyme active site to be a lock and the substrate to be a key. So, if the shape of the lock changes, then the key will no longer fit. This roughly reflects what happens when the active site changes, as the substrate molecule will no longer fit in it.

  1. Factors affecting the activity of potato catalase on hydrogen peroxide.

    So there are more enzymes in the surface, making the reactions faster as they collide with the substrate. Plan/Method: Our coursework is based on a simple reaction between a solution of hydrogen peroxide water and a catalase. This can be shown in an equation: Catalyse -----> 2 H2O2 2H2O +

  2. Investagating the Action of the Enzyme Catalase On the Surface Area of a Potato.

    As you can see from this graph as the amount of catalase enzyme (the surface area of the potato) increases, so does the amount of oxygen produced. The straight line shows that there is a direct link so therefore; we can say that the surface area (or the amount of catalase)

  1. We are investigating how temperature affects enzyme activity

    We count the bubbles to work out the rate of enzyme activity. Fair Testing To obtain a valid set of results, it is important that the experiment is conducted fairly. To make this experiment fair: we have to keep a few factors the same.

  2. How temperature affects enzyme activity?

    I will look at how temperature affects enzyme activity because it is easier to obtain the correct temperature than it is concentration and pH level. In this experiment every other factor, being pressure, pH and enzyme concentration must be kept the same, so that any changes can only be the result of the temperature change.

  1. Investigating the influence of pH on the activity of potato catalase

    This trend is caused because as the hydrogen ion concentration decreases, the solution becomes more alkaline creating conditions similar to the normal environment of potato catalase. The hydrogen ion concentration (pH level), has the effect on the enzyme potato catalase in that it alters the charges on the acidic and

  2. Investigate the factors affecting the activity of the enzyme catalase

    There for I believe that the amount of oxygen will increase as the size of potato increases in size. I think this will happen because the potato contains the enzyme, so as the amount of catalase (enzyme) increases the more active sites there will be for the reaction to take

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work