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Vary an independent factor to see whether it affects the activity of the enzyme Catalase, using Hydrogen Peroxide (H2O2) and a potato solution (which has Catalase in it).

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Introduction

Catalase Investigation By Christopher Stylianou Aim To vary an independent factor to see whether it affects the activity of the enzyme Catalase, using Hydrogen Peroxide (H2O2) and a potato solution (which has Catalase in it). Factors All the independent variables, which could affect the investigation of Catalase, are: * The temperature of the enzyme: By changing the temperature the enzyme is working at, it might affect the activity of the enzyme Catalase. * The potato surface area: By changing the surface area of the potato, this might affect the activity of the enzyme Catalase. * The mass of the potato: By changing the amount of potato used in the experiment, it might affect the activity of the enzyme Catalase. * The concentration of H2O2: By changing the concentration of H2O2, this might affect the activity of the enzyme Catalase. * The concentration of the potato solution: By changing the concentration of the solution by using water, it might affect the activity of the enzyme Catalase. * The temperature of H2O2: By changing the temperature of H2O2 using water baths etc... this might affect the activity of the enzyme Catalase. * The pH of the potato solution: By altering the pH of the potato solution, it could affect the activity of the enzyme Catalase. * The volume of H2O2: By changing the volume of H2O2, it might affect the activity of the enzyme Catalase. ...read more.

Middle

This is because the average optimum temperature of Catalase is around 40 - 45�C. Anything below that temperature will take longer to produce a certain amount of gas and any temperature above this will denature the enzyme, so it will have no affect on the result. I have worked this out using my research above and my general knowledge. I have carried out a preliminary experiment to test out a plan and get an idea where my range should be for the real investigation. I decide to time how long the temperatures 30, 40 and 50�C would take to produce 50ml of Oxygen, using a solution of 5g of potato with 15ml of water, and then reacted with 20ml of Hydrogen Peroxide. Here are my results: Temperature �C Time Taken (mins/sec/10th sec) 30 5:30.45 40 3:31.21 50 4:23.54 The results show that 40�C is the shortest time to produce 50ml of gas. Using these results I can draw a preliminary graph of what the results should look like. I have decided to use a range of 20, 25, 30, 35, 40, 45 and 50�C temperature for the investigation to get a good range of accurate results. I am going to see how long it takes to produce 50ml of Oxygen using 5g of Potato, 15ml of Water and 20ml of Hydrogen Peroxide. Plan: - Apparatus: - Potato Solution: 35g of Potato and 105ml of Water 20ml Hydrogen Peroxide Water baths at 20, 25, 30, 35, 40, 45 and 50�C Burette Burette Clamp Thermometer Stand Timer Conical Flask Delivery tube Food Processor Scalpels Scales (with tray) ...read more.

Conclusion

* Make sure the scales start on 0.00 grams * The temperature of the potato solution is correct and does not change during an experiment. * Make sure the pH of the potato solution does not change as well as it can affect the experiment. To stop this from happening make sure you use the same potato solution for each experiment. Safety: - To make it a fair test you must carry out these precautions: - * Wear Safety Goggles at all times. * Bags and Stalls are put on the sides of the room, out of the way of any experiment. * The normal Science Labs rules must be obeyed. * Be careful with harmful substances. * Watch out for Glass objects, which might get broken. Results: - * = Anomalous Result Experiment 1 Temperature of Catalase (Potato Solution) �C Time taken to produce 50ml of Oxygen (mins/sec/10th sec) 20 7:47.54 25 * 7:03.12 * 30 5:20.19 35 4:00.46 40 3:24.38 45 3:57.56 50 4:24.54 Experiment 2 Temperature of Catalase(Potato Solution) �C Time taken to produce 50ml of Oxygen (mins/sec/10th sec) 20 7:42.01 25 6:21.15 30 5:31.41 35 4:15.06 40 3:26.24 45 3:67.37 50 4:31.46 Experiment 3 (Repeat for Anomalous) Temperature of Catalase(Potato Solution) �C Time taken to produce 50ml of Oxygen (mins/sec/10th sec) 20 - 25 6:32.51 30 - 35 - 40 - 45 - 50 - Average Results Temperature of Catalase(Potato Solution) �C Time taken to produce 50ml of Oxygen (mins/sec/10th sec) 20 7:44.78 25 6:26.85 30 5:26.03 35 4:07.76 40 3:25.31 45 3:62.47 50 4:28.00 ...read more.

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