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We are investigating how temperature affects enzyme activity

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WE ARE INVESTIGATING HOW TEMPERATURE AFFECTS ENZYME ACTIVITY PLANNING Prediction I predict that the higher the temperature the higher the rate of enzyme activity, which would result in a higher number of, bubbles being produced, but the temperature should not get too hot or too cold because the process will slow down again. I predict that this is what the graph will look like: Scientific Explanation The chemical reactions that occur in cells all the time do not happen at random. They are under the control of the nucleus. The nucleus controls which enzymes are produced and it is the enzymes, which directly control the reactions. An Enzyme is a biological catalyst. Enzymes control the rate of chemical reactions, which occur in living organisms. The reactions take place in cells. Enzymes work best under certain conditions of temperature. Enzymes are large proteins. Each enzyme has an active site, which is responsible for controlling one reaction. An enzyme is said to be specific to a reaction. They act as catalysts and change the rate of reactions. Only a small amount of enzyme is needed for a reaction. Generally, as the temperature increases, it causes collisions between reactants and enzymes and the rate of the reaction increases (usually the rate doubles for every 10�C increase in temperature). ...read more.


* Time - Time is a very important factor in this experiment. We as a class decided to observe the enzyme activity for two minutes. It is important that we observe the activity for only two minutes each time we conduct the experiment. Range of results The results that need to be taken are the amount of bubbles that are produced at the end of the two minutes. I am repeating each experiment three times. This is how I am going to lay my table out: TEMPERATURE NUMBER OF BUBBLES 1 NUMBER OF BUBBLES 2 NUMBER OF BUBBLES 3 AVERAGE 5�C 10�c 16�c 22�c 29�c 30�c 35�c 40�c 48�c 50�c 60�c 70�c I will repeat the experiment three times and then I will find out the average amount of bubbles. This will make my evidence more accurate. I may have made a mistake whilst conducting the experiment so by repeating the results three times and finding the average, I am obtaining a more accurate result. Safety Safety is a very important factor when conducting experiments. If we don't pay attention to the safety factors that we need to take into consideration, then someone can get hurt quiet seriously. ...read more.


The anomalies were quiet obvious because when the best-fit line was drawn these points were not within the range of the best-fit line. I think my method was quite efficient and straightforward. It was a clear step-to-step method, which if followed properly would have resulted in a perfect experiment. To improve my method I would give more specific information to make the experiment run as smoothly and as accurately as possible. During the experiment my accuracy of counting the bubbles was not very precise because all the bubbles were not the same size. It was not fair to count two small bubbles as double the value of one large bubble. Next time I could trap the bubbles in a measuring cylinder and measure the volume of gas to give a more accurate reading. This is turn would make my results more reliable to give a fair conclusion. I have acquired a strong and good set of results, which could be used to show somebody: how temperature affects the rate of enzyme activity. To support the evidence I have gained even further I think it would be wiser to repeat the results more often whilst conducting the experiment. Equally I could conduct the same experiment several times and then merge all my results together. 28/11/2002 Prashanthini.S Science Coursework 1 ...read more.

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