• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

What Affects the Enzyme Catalase?

Extracts from this document...

Introduction

Shimon Simon 10SI 10/07/2002 Biology Coursework What Affects the Enzyme Catalase? Background information: An enzyme is a large protein that acts as a biological catalyst and which changes the rate of a reaction. It provides an active site which is an environment where a reaction can take place this is made up of amino acids. The structure and shape of the substrate, the structure and shape of an enzyme and the substance upon which the enzyme works all have to match exactly. This enables the substrate to bind, but it can't do this if the shapes of the two are different. An enzyme itself will not be affected by the reaction and if the reactants are released the enzyme can be bound with a new substrate. The enzyme I am investigating is catalase. Catalase is made in all organisms including yeast cells which will be used for my experiment. Catalase breaks down Hydrogen peroxide into water and oxygen. Hydrogen Peroxide Catalase = Water + Oxygen 2H2O2 H 2O O 2 There are many different factors which affect how catalase works. One of these is the temperature of the substrate. By increasing the temperature I will increase the rate of reaction. This is explained with the use of collision theory. ...read more.

Middle

If the concentration is trebled then so will the rate of reaction. The basis of this prediction can be shown by looking at what the concentration does. A smaller concentration will mean that the molecules of Hydrogen peroxide will be less since there is more water. There will therefore not be many successful collisions between the enzyme and Hydrogen peroxide. By doubling the concentration there will be double the amount of hydrogen peroxide per cm3 less water and so double the amount of successful collisions, double the amount of oxygen and double the speed so half the time due to more successful collisions per second. By increasing the concentration each time by a factor I should be able o observe a constant. This is my preliminary results while using 10cm3 yeast trying each concentration twice as follows: Volume of Oxygen collected (cm3) Concentration of Hydrogen peroxide in total of 20cm3 (Hydrogen Peroxide: water) 5:15 (1/4) 10:10(1/2) 15:5(3/4) 20:0 (1) Time (minutes/seconds) 1st 2nd average 1st 2nd average 1st 2nd average 1st 2nd average 0.30 9 7 8 15 19 17 26 22 24 27 33 30 1 10 12 11 23 23 23 35 33 34 44 46 45 1.30 16 14 15 31 29 30 43 45 44 56 ...read more.

Conclusion

There are plenty of values therefore to make a mathematical analysis. I found that 20cm3 of solution was not enough in my preliminary work. Method: I will fill the plastic tub halfway with water and the 200cm3 measuring cylinder to the brim with water. I shall place the measuring cylinder in the tub with the opening at the bottom making sure no water is lost from the measuring cylinder. I will place one end of the delivery tube in the cylinder and attach the other to the bung. I will then put 10ml of yeast suspension in the conical flask. I shall then have to pour the hydrogen peroxide and water in the flask close the bung and get someone else to start the stop clock. Time permitting I will do this 3 times for each concentration and take an average. This should give me accurate results for which to make my mathematical analysis. I will change only the concentration of hydrogen peroxide and leave all other variables the same therefore making a fair test. I will make sure I do not come in contact with Hydrogen peroxide since when it reacts with water it forms caustic soda. Other then this the experiment is quite safe. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Patterns of Behaviour section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Patterns of Behaviour essays

  1. THE EFFECT OF BILE SALT ON THE ACTION OF THE ENZYME LIPASE

    pH for 4% Bile Trial 1 Trial 2 Trial 3 mean/average 0 10 10 10 10 30 10 9.9 9.8 9.9 60 10 9.9 9.5 9.8 90 9.1 8 8.4 8.5 120 8.8 7.7 8 8.1 150 8.7 7.6 7.7 8 180 8.6 7.5 7.5 7.8 210 8.5 7.4 7.4

  2. Effect Of Substrate Concentration On The Activity Of Catalase

    This molecule is known as the substrate, and is usually specific to the active site of the particular enzyme, which breaks it down. Substrates will not usually fit into any other active sites other than that of the enzyme it is specified to.

  1. The Effect of Catalase in the Breakdown of Hydrogen Peroxide

    Once this is done, the enzyme can no longer fit a substrate, so denatures. In conclusion; a low temperature can cause the enzyme to work exceptionally slow, on the other hand; an environment higher than body temperature can lead to the denaturing of the enzyme.

  2. The effect of concentration on the activity of catalase.

    The volume of buffer solution will remain constant throughout the experiment. The buffer solution will maintian the pH at 6.3 throughout the experiment. A pH of 6.3 is used as catalase works best at acidic conditions. The concentration and volume of hydrogen peroxide will also remain constant.

  1. Enzymes - show how substrate concentration affects the rate of reaction for an enzyme ...

    These types of inhibitors can be permanent for some reaction but also can be temporary hence can be reversed. Substrate Substrate Active site distorted hence Active site substrate cannot bind ENZYME Non-competitive inhibitor binds Non- competitive inhibitor to allosteric site Rate of Reaction From all the factors above, my aim

  2. Investigate the effect of changing substrate concentration on the rate of the reaction between ...

    Potato, or enzyme concentration also needs to be controlled as this will help us investigate how the different amount of substrate (hydrogen peroxide) for the same amount of catalase (in potato) causes change in the reaction rate. Prediction : Based on scientific theory, I can predict that a high substrate

  1. Investigate how temperature affects the rate of reaction of the enzyme catalase on its ...

    However at high temperatures enzymes will begin to denature. This means the attractions holding together the shape of the enzyme will begin to break so the active site loses its unique shape and is unable to react with its substrate. The optimum temperature for most enzymes is 37?C, after this they begin to denature.

  2. Investigate how the concentration of the substrate hydrogen peroxide (H2O2) affects the rate of ...

    The effect of the competitive inhibition can be reversed by increasing the amount of inhibitor. Figure 3 shows the effect of a competitive inhibitor on an enzyme-controlled reaction over a range of different amounts of substrate (to speed up the reaction).

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work