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What affects the rate of an enzyme reaction?

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Introduction

What affects the rate of an enzyme reaction? PLANNING I am going to investigate the speed of reaction when pieces of potato or catalase are put into Hydrogen Peroxide (2H2O2).By doing this I will be able to prove what does effect the rate of an enzyme reaction. In order to get the most out of this experiment I will to begin with do some preliminary work to make sure I know what I'm going to do and the items that I will need to identify about the experiment before I start the investigation. The main things that I will need to find out are as follows: 1. The amount of Hydrogen Peroxide to use 2. The sizes of the potato that will be the best So you see that the preliminary work is really vital as it will help me answer the above queries that I have. The preliminary work was quiet hard as it gave me a taster of what the real experiment will be like. I have found out after doing the preliminary work that the best amount of water is 5cm3. Also the best possible sizes of the potato were 1x1x1, 2x1x1, 3x1x1, 4x1x1, and 5x1x1 (all in centimetres) as all these sizes did fit the test tubes. ...read more.

Middle

Catalase is an enzyme found both in humans and plants. In humans Hydrogen Peroxide is poisonous and builds up during respiration. To decompose this substance quickly the catalase in our liver acts as an biological catalyst to speed up the reaction. Catalase is the fastest ever enzyme in the human body working at nearly 6x105 reaction per minute. In plants Hydrogen Peroxide builds up also during respiration. In this experiment we are going to use potatoes instead of liver because it can be handled more easily. The apparatus that I will be using for this experiment are as follows: * Boiling tubes * Clamp and stand * Hydrogen Peroxide * Knife * Measuring Cylinder * Pipette and pen for liquid method * Potatoes * Ruler * Stop clock * Washing up liquid My method is as follows: 1. First gather all the equipment need for the experiment. Then cut the potato as accurately as you can, using the ruler. 2. Next set up the equipment and measure out the hydrogen peroxide and pour into the boiling tube. 3. After that I will put the washing up liquid using the pipette and then will immediately add the potato. ...read more.

Conclusion

If you increase the concentration of the enzyme this immediately will amplify the number of particles present and as a result boosts the chance of any collisions. EVALUATION In doing the experiment I have come across some problems. The most difficult problem was the measuring of the bubbles. As you can see from the results table the amount of bubbles went into decimals i.e. 2.1 cm3 and therefore it was very crucial that the measurement was read carefully and precisely. The problem above can not affect the results in the big picture. I might have been one tenth of a centimetre more or less but this can minimally affect the graph but the idea will still be the same. Surprisingly there were no odd results. Maybe this was due to the accuracy of my work or maybe it was just luck. The graph has also a line of best fit in which we can see how accurate our results were. The line of best fit goes through three of the points, as this tells us that the results were pretty precise. The other two points are not very far from the line of best fit. This also backs up that the results are pretty precise and accurate. BIOLOGY COURSEWORK M.R 11.1 ...read more.

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