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# What are the best conditions for the germination of green beans?

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Introduction

Kevin Fan 11A What are the best conditions for the germination of green beans? Aim: To plan and find out the optimal amount of water required to germinate green beans. Equipment: a) 4 Petri dishes b) Cotton Wool (as much as needed to entirely cover bottom of Petri dish) c) Water (for Petri dishes; 1 with none (control), 1 with 5ml, 1 with 10ml and 1 with 15ml) d) 12 green beans (3 beans per Petri dish) e) Ruler (For measuring length of root after germination) f) Cellophane tape (for sealing Petri dish, keeping internal conditions constant) g) Labels (identifying which Petri dish was which) Diagram: Dependant variable: Length of root (measured) Independent variable: Amount of water (0ml, 5ml, 10ml, 15ml) Controlled variables: Light intensity + wavelength (All four Petri dishes will be left at the window), temperature (room temperature, ~25oC), carbon dioxide levels (Petri dish is sealed), number of seeds (3 seeds per Petri dish) I will observe and measure: The length of the root after 2 days. I will take the length of all 3 seeds in each Petri dish and record a mean length of root Planned Method: 1) Gather all equipment 2) ...read more.

Middle

0ml (Control) 0.0 0.0 0.0 0.0 0.000 5ml 0.0 0.0 1.5 2.0 0.875 10ml 0.0 2.0 2.0 4.0 2.000 20ml 1.0 3.0 3.0 3.0 2.500 After 2 days (Length of root is measured) Amount of Water Seed 1 (mm) Seed 2 (mm) Seed 3 (mm) Seed 4 (mm) Mean (mm) 0ml (Control) 0.0 0.0 0.0 0.0 0.000 5ml 0.0 1.0 1.0 4.5 1.625 10ml 2.5 4.0 15.0 31.5 13.250 20ml 7.0 17.5 19.0 27.0 17.625 Conclusion: There really isn't an optimum amount of water to germinate green beans; the more the better. As proven by the results, the control (with no water) did not germinate at all, and the more water provided, the longer the root of the germination plant. With 5ml of water, the average root grew 0.875mm after two days, but with 10ml, the length of the average root is more than doubled (13.625mm). The seeds in Petri dish 2 germinated markedly slower, and even after two days the average length of root did not even exceed the average length posed by the seeds in petri dish 3 after one day. ...read more.

Conclusion

Diagrams of this are shown on the right: - Amounts of cotton wool used for each Petri dish were not equal. Although this is quite a minor problem, it can easily be solved by first weighing the amount of cotton wool put into each dish and making sure that the weight of cotton wool in all three Petri dishes are the same. - During the two days over which the experiment was performed over, the seeds could have been displaced to an area with completely different conditions. This problem probably resulted in a seed from Petri dish 2 that failed to germinate even after two days. A method to solve this is possibly to nip off bits of cotton wool so a little hole for each seed is effectively made. Below are some things that were implemented well: - Orientation labels were used. This enabled me to measure the same green bean on both days. - Petri dishes were sealed properly This would ensure that many variables are controlled eg. temperature, CO2 levels, moisture etc. - Four beans were used for each Petri dish. This ensures variable control (ie. Number of seeds) and four seeds is manageable yet informative. ...read more.

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