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What I will be investigating is the average height of foam that is produced when catalase reacts with hydrogen peroxide.

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Introduction

Biology Coursework Enzymes are biological catalysts that speed up the rates or reactions by both breaking down and building up compounds, enzymes are 3-D globular proteins. A catalyst is any substance which makes a chemical reaction go faster, without being changed. A catalyst can be used over again in a chemical reaction: it does not get used up. Enzymes are very much the same except that they are easily denatured. The active site of an enzyme is where the substrates and bind and undergo a chemical reaction. The active site of an enzyme is complementary in shape to the substrate, they work together like a "lock and key", (diagrams shown above and right). This explains the process of enzyme specificity. Each enzyme fits only one substrate (or a very small number) therefore there is a different enzyme for each reaction. There are a few variables that effect enzyme activity such as the pH of the enzyme, the surface area of the enzyme, the substrate being used and finally the temperature which is what we are aiming to investigate. When enzymes are at a low temperature activity is low because the substrates and enzyme molecules move slowly and there is fewer collisions between them. ...read more.

Middle

Apparatus Potato Hydrogen Peroxide Detergent White Tile Cork borer no.4 and 5 Test Tubes Test Tube Rack Knife Measuring Cylinder Beaker Stop Clock Ruler Water Baths Thermometer Forceps Distilled Water Dropper Safety There are a few safety precautions that need to be taken such as safety glasses being worn to protect the persons eyes. Hydrogen peroxide is an irritant so it is important that It does not come I contact with the skin as it could cause burns. It could also seriously damage your eyes so that is why it is important to wear safety glasses at all times. Method ==> Prepare a table to record results of your findings ==> Prepare a potato cylinder, which is used by putting cork borer 5 into a piece of potato and then pushing the piece out using cork borer 4 ==> Cut the potato cylinder so it is 1 cm in length ==> Prepare 3 test tubes each containing 10cm� of Hydrogen Peroxide ==> Heat the potato and Hydrogen Peroxide separately to the desired temperature in the water bath. ==> Add a drop of detergent to the hydrogen peroxide and then add the potato cylinder ==> Time the experiment ==> Measure the height of foam that is produced when catalase reacts with Hydrogen Peroxide of 10cm3. ...read more.

Conclusion

Also I would repeat the experiment using smaller temperature intervals to determine a more accurate optimum temperature, possibly going up in 2�C instead of the 10�C that we done. I predicted that the optimum temperature would be roughly around 40?C, I found that here my results agreed with my prediction. I predicted that the enzyme would completely denature at 60?C, but I found that this was not the case as I can see from my graph that there was still some enzyme activity at 60?C. This could be because not all the potato was heated to 60?C. The centre of the potato may have been lower than 60?C meaning a reaction could still occur as not all the bonds in the enzyme had denatured. The results did not support a firm conclusion, as it was only repeated twice because I did not have to repeat it more times. Ideally it should have been repeated ten times. When I compared my results with my classmates, the results varied as some peoples optimum temperature was 30�C and others was 40�C, but everyone got the same trend out of the same experiment under the same tightly controlled conditions. The trend was that as temperature rises to the rate optimum, rate of reaction increases and as the temperature passes the optimum the rate of reaction decreases. ?? ?? ?? ?? 1 ...read more.

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