With increasing concentration of urease, the time it takes to neutralize the ethanoic acid will decrease.

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A study of urease

Aim:

To study the effect of varying concentrations of urease on the decomposition of urea.

Hypothesis:

With increasing concentration of urease, the time it takes to neutralize the ethanoic acid will decrease.

Justification of hypothesis:

The concentration of substrate molecules, in this case urea, stays constant. As enzyme concentration increases more collisions occur with the substrate molecules and so more reactions occur. As more reactions occur, the concentration of ammonia increases and the faster will the ethanoic acid be neutralized.  

Variables:

Independent variable: The concentration of urease in the solution

Dependent variable: Rate of action of urease

Controlled variables: Concentration of urea in the solution, concentration of ethanoic acid in the solution, temperature of the solutions, kind of indicator used.

Data collection:

Total volume of solution in test tube: 9.0 mL ± 0.1 mL

Concentration of ethanoic acid added to test tube: 1.0 M

Volume of ethanoic acid added to test tube: 2 mL ± 0.1mL

Concentration of urea solution added to test tube: 1%

Volume of urea solution added to test tube: 5 mL ± 0.1 mL

Indicator used: BHD universal indicator

Color of BHD universal indicator at pH values below 7: pink

Color of BHD universal indicator at pH values above 7: green

Concentration of urease solution added to test tube: 5%

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             Table showing my personal raw data:

Table showing the raw data of the entire class:

Data processing:

Table showing the average time taken for the neutralization point to occur

Sample calculations :

Average time taken for the neutralization point to occur:

(data of group 1 + group 2 + group 3 + group 4) / (number of groups)

For example, for the average time for 3mL of urease added:

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