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Thin Layer Chromatography

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Introduction

Thin Layer Chromatography Plant Pigments Aj Klatch Kyle Tisi Mr. Edmondson AP Chemistry Table: 9/10 2/4/01 2/3 I. Abstract The purpose of this lab was to determine the rate of flow values (Rf=distance of component/distance of solvent) for certain pigments found in chlorophyll and carotene. For the chlorophyll, we observed the following five colors: yellow, orange, dark green, green, and light green. For the cartenoid, we observed the following five colors: yellow, auburn, purple, pink, and red. The solvent in both of our experiments traveled a distance of 6.8cm. We obtained the following Rf values for the chlorophyll. For yellow, Rf=0.89; for orange, Rf=0.78; for dark green, Rf=0.51; for green, Rf=0.49; and for light green, Rf=0.44. We obtained the following Rf values for the carotene. For yellow, Rf=0.89; for auburn, Rf=0.81; for purple, Rf=0.69; for pink Rf=0.51; and for red, Rf=0.49. The second part of this lab dealt with statistical treatment of four of our lowest Rf values of chlorophyll compared to the rest of the class. Our personal S.D.M. value for the chlorophyll was 0.080. The S.D.M. for the lowest level of the class' Rf value was 0.021. The S.D.M. for the second level of the class' Rf value was 0.026. The S.D.M. ...read more.

Middle

Solvent 6.8cm N/A Yellow 6.1cm .89 Auburn 5.5cm .81 Purple 4.7cm .69 Pink 3.5cm .51 Red 3.3cm .49 c. Statistics i. Our Chlorophyll X M R |(x-m)| R2 ? = (? R2/(N-1))1/2 S.D.M. (?/N1/2) .44 .62 .18 .032 .19 .084 .49 .13 .017 .51 .11 .012 .78 .16 .026 .89 .27 .073 ii. Class Chlorophyll - Lowest Values X M R |(x-m)| R2 ? = (? R2/(N-1))1/2 S.D.M. (?/N1/2) .38 .45 .070 .0044 .050 .021 .43 .020 .00040 .44 .010 .00010 .45 0 0 .51 .060 .0036 .51 .060 .0036 iii. Class Chlorophyll - Second Lowest Values X M R |(x-m)| R2 ? = (? R2/(N-1))1/2 S.D.M. (?/N1/2) .42 .48 .060 .0036 .065 .027 .44 .040 .0016 .45 .030 .00090 .46 .020 .00040 .56 .080 .0064 .57 .090 .0081 iv. Class Chlorophyll - Third Lowest Values X M R |(x-m)| R2 ? = (? R2/(N-1))1/2 S.D.M. (?/N1/2) .43 .57 .14 .020 .144 .059 .51 .06 .0036 .51 .06 .0036 .52 .05 .0025 .60 .03 .00090 .84 .17 .029 v. Class Chlorophyll - Highest Values X M R |(x-m)| R2 ? = (? R2/(N-1))1/2 S.D.M. (?/N1/2) ...read more.

Conclusion

We obtained the following Rf values for the carotene. For yellow, Rf=0.89; for auburn, Rf=0.81; for purple, Rf=0.69; for pink Rf=0.51; and for red, Rf=0.49. The second part of this lab dealt with statistical treatment of four of our lowest Rf values of chlorophyll compared to the rest of the class. Our personal S.D.M. value for the chlorophyll was 0.080. The S.D.M. for the lowest level of the class' Rf value was 0.021. The S.D.M. for the second level of the class' Rf value was 0.026. The S.D.M. for the third level of the class' Rf value was 0.058. The S.D.M. for the highest level of the class' Rf value was 0.068. The mean value from lowest to highest of the class' Rf values are as follows: .45, .48, .57, and .79. Something to note about the data is the comparison of the S.D.M. values. Our personal S.D.M. values was slightly higher then the S.D.M. values calculated for each section of the class. However, our S.D.M. is calculated with all of the values we received instead of just one batch of data. Also, it appears as though as the numbers grow larger, the deviance of the class grows along with it which means that as numbers are greater the class becomes less accurate. 2 ...read more.

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