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An Investigation on the Effect of the Concentration of Hydrogen Peroxide on the rate of Reaction.

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Introduction

Biology Lab Report: An Investigation on the Effect of the Concentration of Hydrogen Peroxide on the rate of Reaction. Introduction: The aim of this experiment is to investigate effect of the concentration of hydrogen peroxide on the rate of its reaction with the enzyme Catalase. I will investigate the rate of reaction by measuring the volume of substrate gas (oxygen) given off during the reaction of different concentrations of hydrogen peroxide catalyzed by 0.5% catalase in a minute. From the results of this experiment we should be able to observe a positive or negative correlation between the rate of reaction and the concentration of hydrogen peroxide as well as the peak rate of catalysis of 0.5% catalase. Hydrogen peroxide is a natural byproduct of many metabolic processes, including some of those that take place in the human body. Since hydrogen peroxide is an unstable oxidizer it may be harmful to the cell and must be quickly broken down into water and oxygen which harmless. The enzyme Catalase is the primary enzyme used by every known animal in every organ to catalyze the reaction. It is important for a cell to produce enough catalase (higher than peak turnover rate) to maintain high efficiency to avoid damage from hydrogen peroxide. I will seek to find this peak turnover rate by increasing the concentration of the substrate until maximum efficiency is reached and rate of reaction ceases to increase. ...read more.

Middle

6. Temperature For the most accurate results, the entire experiment should take place under thermostatic conditions because temperature is a factor in the rate of reaction. I did my experiment at room temperature which is controlled by a thermostat. Uncontrolled Variables: 1. Quality of chemicals and equipment As I only have access to shared school equipment and chemicals, its quality and cleanliness could not be completely guaranteed. Though this could be offset by purchasing new equipment for the best results, the costs of such undertaking outweigh the potential improvement of accuracy. Apparatus: * 50 cm3 Glass syringe (to measure the gas given off) * Rubber tubing connected stopper (to connect conical flask to syringe) * 50 cm3 Conical flask * Boss and clamp stand * 25 cm3 Measuring cylinder * Stop watch * Safety Equipment 1. Safety goggles 2. lab coat 3. gloves * Chemicals 1. 10cm3 0.5% Catalase solution 2. 25 cm3 of 5%, 10%, 15%, 20% Hydrogen Peroxide solution 3. 25 cm3 of distilled water for control experiment Method: 1. Set up equipment with syringe held horizontally by the clamp and rubber tubing connecting syringe to the stopper. Check for leaks by blowing into system and watching for escaping air. 2. First prepare the control experiment by measuring 25 cm3 of distilled water with measuring cylinder and pour into conical flask. ...read more.

Conclusion

Our apparatus included a gas syringe which meant that there could be a lot of problems to be expected and was right, the plunger of the syringe often got stuck and on the different days we might get different syringes meaning our results were not very accurate and consistent. Lastly, human error must be considered as the experiment was carried out by humans and mistakes are inevitable. Many of these factors could be eliminated or minimized if the following precautions are taken. The environmental factors could be eliminated in the future by immersing the chemicals in a water bath at a fixed temperature to make sure the reagents are at the same temperature when reacting, as well as by carrying out the experiment at the same time of day each day so that the room is aproximately the same temperature. To minimize light exposure during the experiment the lights should be turned down when doing the experiment and to promptly carry out the experiment, not letting it break down in the light. This could be eliminated by using high grade equipement and using the same equipment each time to ensure reliable and comparable results. This experiment requires a lot of accurate measuring for the experiment to be reliable however human error may compromise this. This can be reduced by having more skilled people to help in the experiment and everyone having their own job as well as taking care when taking measurements. ?? ?? ?? ?? Jim Chun-Chen Lu (001407 033) Biology HL Taipei European School (001407) ...read more.

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